PRELP Antibodies

HAF016). A specific band was detected for PRELP at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for PRELP at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human liver tissue and human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PRELP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6447) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PRELP at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

6447-PR) inhibits TRANCE-induced osteoclast differentiation in the RAW 264.7 mouse monocyte/macrophage cell line in a dose-dependent manner (orange line), as measured by the Tartrate-resistant acid phosphatase activity assay. Under these conditions, TRANCE-induced osteoclast differentiation inhibited by Recombinant Human PRELP (5 µg/ml) is neutralized (green line) by increasing concentrations of Mouse Anti-Human PRELP Monoclonal Antibody (Catalog # MAB64471) in the presence of 5 ng/mL Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TEC) and 20 ng/mL Recombinant Mouse M-CSF (Catalog # 416-ML). The ND₅₀ is typically 0.25-1.25 µg/mL." class="big_lightbox"> 6447-PR) inhibits TRANCE-induced osteoclast differentiation in the RAW 264.7 mouse monocyte/macrophage cell line in a dose-dependent manner (orange line), as measured by the Tartrate-resistant acid phosphatase activity assay. Under these conditions, TRANCE-induced osteoclast differentiation inhibited by Recombinant Human PRELP (5 µg/ml) is neutralized (green line) by increasing concentrations of Mouse Anti-Human PRELP Monoclonal Antibody (Catalog # MAB64471) in the presence of 5 ng/mL Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TEC) and 20 ng/mL Recombinant Mouse M-CSF (Catalog # 416-ML). The ND₅₀ is typically 0.25-1.25 µg/mL." alt="Recombinant Human PRELP (Catalog # 6447-PR) inhibits TRANCE-induced osteoclast differentiation in the RAW 264.7 mouse monocyte/macrophage cell line in a dose-dependent manner (orange line), as measured by the Tartrate-resistant acid phosphatase activity assay. Under these conditions, TRANCE-induced osteoclast differentiation inhibited by Recombinant Human PRELP (5 µg/ml) is neutralized (green line) by increasing concentrations of Mouse Anti-Human PRELP Monoclonal Antibody (Catalog # MAB64471) in the presence of 5 ng/mL Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TEC) and 20 ng/mL Recombinant Mouse M-CSF (Catalog # 416-ML). The ND₅₀ is typically 0.25-1.25 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="PRELP was detected in immersion fixed human mesenchymal stem cells differentiated into chondrocytes using Mouse Anti-Human PRELP Monoclonal Antibody (Catalog # MAB6447) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />