Pregnancy Zone Protein Antibody

Western blot shows human pregnancy serum. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Pregnancy Zone Protein/PZP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8280) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pregnancy Zone Protein/PZP at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

Pregnancy Zone Protein Antibody Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived recombinant human Pregnancy Zone Protein/PZP
  Thr26-Val1482
  Accession # P20742
• Specificity: Detects human Pregnancy Zone Protein/PZP in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human A2ML1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PZP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pregnancy Zone Protein Antibody

• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6
• CPAMD6
• Pregnancy Zone Protein
• pregnancy-zone protein
• PZP

Background

Pregnancy zone protein (PZP, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6) is a 360 kDa secreted homodimeric glycoprotein, PZP monomer contains a region of decisive functional importance i.e. the bait region. This region serves as a substrate to a variety of proteinases, and cleavage in this domain causes a conformational change of the molecule. Human PZP is a member of the protease inhibitor I39 (alpha-2-macroglobulin) family of proteins.  Highest expression of human PZP is reported in liver, medium expression in ovary, heart and stomach. Also this protein is found in plasma.  Over aa 26-1482, human PZP is 56% aa identical to mouse PZP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PZP
• Uniprot:
  • P20742()