Pregnancy Zone Protein Antibody (909813) [Unconjugated]

Western blot shows lysate of human pregnancy sera. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Pregnancy Zone Protein/PZP Monoclonal Antibody (Catalog # MAB8280) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Pregnancy Zone Protein/PZP at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Recombinant Human Pregnancy Zone Protein/PZP protein was serially diluted 2-fold and captured by Mouse Anti-Human Pregnancy Zone Protein/PZP Monoclonal Antibody (Catalog # MAB82801) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Pregnancy Zone Protein/PZP Monoclonal Antibody (Catalog # MAB8280) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 909813
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Pregnancy Zone Protein Antibody (909813) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human embryonic kidney cell line HEK293-derived recombinant human Pregnancy Zone Protein/PZP
  Thr26-Val1482
  Accession # P20742
• Specificity: Detects human Pregnancy Zone Protein/PZP in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: PZP
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pregnancy Zone Protein Antibody (909813) [Unconjugated]

• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6
• CPAMD6
• Pregnancy Zone Protein
• pregnancy-zone protein
• PZP

Background

Pregnancy Zone Protein (PZP), also known as C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6, is a 360 kDa secreted homodimeric glycoprotein, PZP monomer contains a region of decisive functional importance i.e. the bait region. This region serves as a substrate to a variety of proteinases, and cleavage in this domain causes a conformational change of the molecule. Human PZP is a member of the protease inhibitor I39 (alpha-2-macroglobulin) family of proteins.  Highest expression of human PZP is reported in liver, medium expression in ovary, heart and stomach. Also, this protein is found in plasma.  Over amino acids (aa) 26-1482, human PZP shares 56% aa sequence identity with mouse PZP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PZP
• Uniprot:
  • P20742()