![Western Blot: Pref-1/DLK1/FA1 Antibody (PF105B) [NBP2-80044] - Western blot analysis using anti-DLK1 (mouse), mAb (PF105B) at 1:2'000 dilution.Panel A)1: Mouse DLK1 Fc-protein.2: Human DLK1 Fc-protein.3: Mouse DLL1 Fc-protein.4: Mouse DLL4 Fc protein.5: Mouse Jagged-1 Fc-protein.6: Unrelated Fc-protein (negative control).Panel B)1: Mouse DLK1 Fc-protein.2: Mouse DLL1 Fc-protein.3: Human DLK1 (ED) (FLAG(R)-tagged).4: 3T3L1 cell lysate.](http://images.novusbio.com/fullsize2/Pref-1-DLK1-FA1-Antibody-(PF105B)-Western-Blot-NBP2-80044-img0001.jpg "Western Blot: Pref-1/DLK1/FA1 Antibody (PF105B) [NBP2-80044] - Western blot analysis using anti-DLK1 (mouse), mAb (PF105B) at 1:2'000 dilution.Panel A)1: Mouse DLK1 Fc-protein.2: Human DLK1 Fc-protein.3: Mouse DLL1 Fc-protein.4: Mouse DLL4 Fc protein.5: Mouse Jagged-1 Fc-protein.6: Unrelated Fc-protein (negative control).Panel B)1: Mouse DLK1 Fc-protein.2: Mouse DLL1 Fc-protein.3: Human DLK1 (ED) (FLAG(R)-tagged).4: 3T3L1 cell lysate.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA |
| Clone | PF105B |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant mouse Pref-1/DLK1/FA1 (extracellular domain). |
| Specificity | Recognizes mouse Pref-1/DLK1/FA1. Does not cross-react with human Pref-1/DLK1/FA1. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | DLK1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um-filtered solution in PBS, pH 7.4 |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
![Immunohistochemistry-Paraffin: Notch-1 Antibody (mN1A) [NB100-78486] - Analysis of FFPE human pancreatic cancer using 1:10 dilution of Notch-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in epithelial cells was observed. Staining was performed by Histowiz.](http://images.novusbio.com/images2/Notch-1-Antibody-(mN1A)-Immunohistochemistry-Paraffin-NB100-78486-img0010.jpg)
![Knockout Validated: Notch-1 Antibody (mN1A) [NB100-78486] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Notch-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/mL of Mouse Anti-Human Notch-1 Monoclonal Antibody (Catalog # NB100-78486) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Notch-1 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/Notch-1-Antibody-(mN1A)-Knockout-Validated-NB100-78486-img0011.jpg)
![Flow Cytometry: Notch-1 Antibody (mN1A) [NB100-78486] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and MCF-7 (B) cells using Notch1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/images2/Notch-1-Antibody-(mN1A)-Flow-Cytometry-NB100-78486-img0007.jpg)
![Western Blot: MAP3K13 Antibody [NBP1-81272] - Analysis in human cell line SK-BR-3.](http://images.novusbio.com/images2/MAP3K13-Antibody-Western-Blot-NBP1-81272-img0009.jpg)
![Immunohistochemistry-Paraffin: MAP3K13 Antibody [NBP1-81272] - Staining of human duodenum shows strong membranous positivity in glandular cells.](http://images.novusbio.com/images2/MAP3K13-Antibody-Immunohistochemistry-Paraffin-NBP1-81272-img0010.jpg)
![Immunohistochemistry-Paraffin: MAP3K13 Antibody [NBP1-81272] - Staining of human kidney shows strong membranous positivity in cells in tubules.](http://images.novusbio.com/images2/MAP3K13-Antibody-Immunohistochemistry-Paraffin-NBP1-81272-img0011.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for Pref-1/DLK1/FA1 Antibody (NBP2-80044)Discover more about diseases related to Pref-1/DLK1/FA1 Antibody (NBP2-80044).
| Pathways for Pref-1/DLK1/FA1 Antibody (NBP2-80044)View related products by pathway.
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PTMs for Pref-1/DLK1/FA1 Antibody (NBP2-80044)Learn more about PTMs related to Pref-1/DLK1/FA1 Antibody (NBP2-80044).
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DLK1 |
![Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.](http://images.novusbio.com/images2/PPAR-gamma-NR1C3-Antibody-Western-Blot-NBP2-22106-img0003.jpg)
![Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.](http://images.novusbio.com/images2/PPAR-gamma-Antibody-Immunohistochemistry-Paraffin-NBP2-22106-img0001.jpg)
![Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.](http://images.novusbio.com/images2/PPAR-gamma-Antibody-Western-Blot-NBP2-22106-img0002.jpg)
![Western Blot: MAP3K12 Antibody [NBP2-17218] - A. 20 ug mouse embryonic stem cell whole cell lysate/extract 7.5 % SDS-PAGEDLK/MAP3K12 antibody dilution: 1:1000](http://images.novusbio.com/images2/MAP3K12-Antibody-Western-Blot-NBP2-17218-img0015.jpg)
![Western Blot: MAP3K12 Antibody [NBP2-17218] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody diluted at 1:1000.](http://images.novusbio.com/images2/MAP3K12-Antibody-Western-Blot-NBP2-17218-img0008.jpg)
![Immunohistochemistry-Paraffin: MAP3K12 Antibody [NBP2-17218] - Analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/images2/MAP3K12-Antibody-Immunohistochemistry-Paraffin-NBP2-17218-img0005.jpg)
![Flow Cytometry: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of Hela cells with ERK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/images2/ERK2-Antibody-(SZ25-01)-Flow-Cytometry-NBP2-67360-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/ERK2-Antibody-(SZ25-01)-Immunocytochemistry-Immunofluorescence-NBP2-67360-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/ERK2-Antibody-(SZ25-01)-Immunocytochemistry-Immunofluorescence-NBP2-67360-img0003.jpg)
![Western Blot: HES-1 Antibody (OTI4H1) [NBP1-47791] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1 (1:500).](http://images.novusbio.com/images2/HES-1-Antibody-(OTI4H1)-Western-Blot-NBP1-47791-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hes1.](http://images.novusbio.com/images2/HES-1-Antibody-(OTI4H1)-Immunocytochemistry-Immunofluorescence-NBP1-47791-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791] - HeLa cells using anti-HES1 mouse monoclonal antibody.](http://images.novusbio.com/images2/HES-1-Antibody-(OTI4H1)-Immunocytochemistry-Immunofluorescence-NBP1-47791-img0010.jpg)
![Western Blot: Goat anti-Rat IgG (H+L) Secondary Antibody [HRP] [NB7115] - Detection of DDK in SUSD3 overexpression lysate using anti-DYKDDDDK Epitope Tag antibody (cat.# NBP1-06712) followed by Goat anti-Rat IgG (H+L) Secondary Antibody [HRP]. Image from verified customer review.](http://images.novusbio.com/images2/Goat-anti-Rat-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7115-img0005.jpg "Western Blot: Goat anti-Rat IgG (H+L) Secondary Antibody [HRP] [NB7115] - Detection of DDK in SUSD3 overexpression lysate using anti-DYKDDDDK Epitope Tag antibody (cat.# NBP1-06712) followed by Goat anti-Rat IgG (H+L) Secondary Antibody [HRP]. Image from verified customer review.")
