Pref-1/DLK1/FA1 Antibody (211309) [Alexa Fluor® 488]

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Pref-1/DLK1/FA1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1144G, filled histogram) or isotype control antibody (IC0041G, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

• Reactivity: Hu
• Applications: Flow
• Clone: 211309
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Pref-1/DLK1/FA1 Antibody (211309) [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Pref-1/DLK1/FA1 long isoform
  Ala24-Pro297 (with Arg248Pro and Lys295Ser substitutions)
  Accession # P80370
• Specificity: Detects human Pref‑1/DLK1/FA1 in direct ELISAs and Western blots. Shows approximately 50% cross-reactivity with recombinant mouse Pref‑1/DLK1/FA1.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DLK1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pref-1/DLK1/FA1 Antibody (211309) [Alexa Fluor® 488]

• delta-like 1 homolog (Drosophila)
• DLK
• DLK1
• DLK-1
• FA1
• FA1fetal antigen 1
• pG2
• pG2delta-like homolog (Drosophila)
• preadipocyte factor 1
• Pref1
• Pref-1
• protein delta homolog 1
• secredeltin
• ZOG

Background

Pref-1 (Preadipocyte Factor 1), also known as DLK1 and FA1, is a 58-65 kDa member of the Notch/Serrata/Delta family of proteins.  It is expressed in prechondrocytes and preadipocytes and appears to block progenitor cell differentiation into mature cell lineages.  Mature human Pref-1 is a 360 amino acid (aa) type I transmembrane N- and O-linked glycoprotein.  It contains a 280 aa extracellular region (aa 24-303), a 24 aa transmembrane segment (aa 304-327), and a 56 aa cytoplasmic domain (aa 328-383).  The extracellular region contains six EGF-like domains, and undergoes proteolytic cleavage to generate a bioactive 50 kDa fragment, plus three 25-31 kDa fragments that show no activity.  There are multiple potential splice variants.  One shows a deletion of aa 229-301, a second possesses a six aa substitution for aa 1-52, a third shows a deletion of aa 210-277, while a fourth contains a six aa substitution for aa 207-383.  Over aa 24-297, human Pref-1 shares 82% aa identity with mouse Pref-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pref-1/DLK1/FA1 Antibody (FAB1144G)(2)

Publications using FAB1144G

• Mariniello, K;Pittaway, JFH;Altieri, B;Borges, KS;Hadjidemetriou, I;Ribeiro, C;Ruiz-Babot, G;Lim, JA;Foster, J;Cleaver, J;Sosabowski, J;Rahman, N;Doroszko, M;Hantel, C;Sigala, S;Abate, A;Tamburello, M;Kiseljak-Vassiliades, K;Wierman, M;Parvanta, L;Abdel-Aziz, TE;Chung, TT;Marco, AD;Palazzo, F;Gomez-Sanchez, CE;Taylor, DR;Rayner, O;Ronchi, CL;Gaston-Massuet, C;Sbiera, S;Drake, WM;Rognoni, E;Kroiss, M;Breault, DT;Fassnacht, M;Guasti, L; Dlk1 is a novel adrenocortical stem/progenitor cell marker that predicts malignancy in adrenocortical carcinoma bioRxiv : the preprint server for biology 2024-08-22 [PMID: 39229217] (FACS, Human)
• Y Kieffer, HR Hocine, G Gentric, F Pelon, C Bernard, B Bourachot, S Lameiras, L Albergante, C Bonneau, A Guyard, K Tarte, A Zinovyev, S Baulande, G Zalcman, A Vincent-Sa, F Mechta-Gri Single-cell analysis reveals fibroblast clusters linked to immunotherapy resistance in cancer Cancer Discov, 2020-05-20;0(0):. 2020-05-20 [PMID: 32434947] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: DLK1
• Uniprot:
  • P80370()