PRDM4 Antibody (RAB-C367) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: PRDM4 Antibody (RAB-C367) - Chimeric - Azide and BSA Free [NBP3-20139] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C367 (NBP3-20139) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20139, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Immunocytochemistry/Immunofluorescence: PRDM4 Antibody (RAB-C367) - Chimeric - Azide and BSA Free [NBP3-20139] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C367 (NBP3-20139) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20139, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IP, ChIP, MS
• Clone: RAB-C367
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

PRDM4 Antibody (RAB-C367) - Chimeric - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing PRDM4 protein under non-denaturing conditions.
• Epitope: This antibody binds to a folded domain, amino acids 390-540.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRDM4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Mass Spectrometry
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PRDM4 Antibody (RAB-C367) - Chimeric - Azide and BSA Free

• MGC45046
• PFM1PR domain-containing protein 4
• PR domain containing 4
• PR domain zinc finger protein 4
• PR-domain zinc-finger protein PFM1

Background

PRDM4/PFM1 may function as a transcription factor involved in cell differentiation. It contains 6 C2H2-type zinc fingers and is expressed in many tissues. Highly expressed in ovary, testis, pancreas, brain, heart and prostate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRDM4