PRDM16/MEL1 Antibody

Flow Cytometry: PRDM16/MEL1 Antibody [NB100-1088] - Analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PRDM16/MEL1 Antibody Summary

• Description: Novus Biologicals Goat PRDM16/MEL1 Antibody (NB100-1088) is a polyclonal antibody validated for use in WB, ELISA and Flow. Anti-PRDM16/MEL1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-TSESGAFHPINHL corresponding to C-Terminus according to NP_071397.2, NP_955533.1.
• Localization: Nuclear
• Specificity: This antibody is expected to recognise both reported isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRDM16
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot
• Application Notes: Use in Western blot reported in scientific literature (PMID: 23221344).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PRDM16/MEL1 Antibody

• MDS1/EVI1-like gene 1
• MEL1
• MEL1KIAA1675PFM13MGC166915
• PFM13
• PR domain containing 16
• PR domain zinc finger protein 16
• PR domain-containing protein 16
• PRDM16
• Transcription factor MEL1

Background

The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36,q21)-positive MDS/AML. The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal PR domain. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRDM16/MEL1 Antibody (NB100-1088)(2)

Publications using NB100-1088

• Stanford KI, Middelbeek RJ, Townsend KL et al. Brown adipose tissue regulates glucose homeostasis and insulin sensitivity J Clin Invest 2013-01-02 [PMID: 23221344] (WB, Mouse)
• Mochizuki N, Shimizu S, Nagasawa T et al. A novel gene, MEL1, mapped to 1p36.3 is highly homologous to the MDS1/EVI1 gene and is transcriptionally activated in t(1;3)(p36;q21)-positive leukemia cells. Blood 2000-11-01 [PMID: 11050005]

Bioinformatics

• Gene Symbol: PRDM16
• Entrez:
  • 63976(Human)
• Uniprot:
  • Q9HAZ2(Human)