PRAME Antibody - BSA Free

Western Blot: PRAME Antibody [NBP1-85418] - Analysis in human cell lines U2OS and SK-MEL-30 using Anti-PRAME antibody. Corresponding PRAME RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PRAME Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LLLSHIHASSYISPEKEEQYIAQFTSQFLSLQCLQALYVDSLFFLRGRLDQLLRHVMNPLETLSITNCRLSEGDVMHLSQSPSVSQLSVLSLSGVMLTDVSPEPLQALLERASATLQDLVFDECGI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRAME
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.04-0.4 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PRAME Antibody - BSA Free

• cancer/testis antigen 130
• MAPEOIP4CT130
• melanoma antigen preferentially expressed in tumors
• OIP-4
• Opa-interacting protein 4
• Opa-interacting protein OIP4
• preferentially expressed antigen in melanoma
• Preferentially expressed antigen of melanoma

Background

PRAME encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Recent data from Epping et.al., (Cell, Vol. 122: 835) suggest that overexpression of PRAME frequently observed in human cancers confers growth or survival advantages by antagonizing RAR signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRAME Antibody (NBP1-85418)(2)

Publications using NBP1-85418

• See S, Smith S, Finkelman B et al. The role of PRAME and NY-ESO-1 as potential therapeutic and prognostic biomarkers in triple-negative breast carcinomas Pathology - Research and Practice 2022-12-01 [PMID: 36603407]
• Lam RA, Tien TZ, Joseph CR et al. Cancer-Testis Antigens in Triple-Negative Breast Cancer: Role and Potential Utility in Clinical Practice Cancers (Basel) 2021-07-31 [PMID: 34359776]

Bioinformatics

• Gene Symbol: PRAME
• Entrez:
  • 23532(Human)
• Uniprot:
  • P78395(Human)