PRAME Antibody (EPR20330)

• Reactivity: Hu
• Applications: IHC
• Clone: EPR20330
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PRAME Antibody (EPR20330) Summary

• Description: Novus Biologicals Rabbit PRAME Antibody (EPR20330) (NBP3-45107) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fragment within Human PRAME aa 100 to the C-terminus. The exact sequence is proprietary
• Specificity: PRAME (Preferentially expressed Antigen in Melanoma) is a tumorassociated antigen and is a member of the family of cancer testis antigens (CTA). PRAME is expressed in malignant cells, including leukaemias, Hodgkin's lymphoma, breast cancer, and primary and metastatic melanomas. PRAME has low or no expression in normal tissues except for in testis, ovary, placenta, adrenals and endometrium.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRAME
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS (pH 7.4), Tween-20, 0.2% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for PRAME Antibody (EPR20330)

• cancer/testis antigen 130
• MAPEOIP4CT130
• melanoma antigen preferentially expressed in tumors
• OIP-4
• Opa-interacting protein 4
• Opa-interacting protein OIP4
• preferentially expressed antigen in melanoma
• Preferentially expressed antigen of melanoma

Background

PRAME encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Recent data from Epping et.al., (Cell, Vol. 122: 835) suggest that overexpression of PRAME frequently observed in human cancers confers growth or survival advantages by antagonizing RAR signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRAME