PRAM1 Antibody

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Western Blot: PRAM1 Antibody [NB100-821] - Analysis of PRAM1 in Jurkat lysate using this antibody at 2 ug/ ml. Primary incubated for 12 hour. Detected using chemiluminescence.

Product Details

Summary
Reactivity Hu, PoSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PRAM1 Antibody Summary

Immunogen
Peptide with sequence DFCDPLENQPLPLGR corresponding to C-Terminus according to NP_115528.4.
Predicted Species
Porcine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PRAM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1:100 - 1:2000
Application Notes
WB: Approx. 90-100 kDa band observed in Jurkat lysates. Overnight incubation is recommended with this antibody. An 79 kDa band of PRAM-1 (predicted MW of 79 kDa band) and detected a migration with an apparent MW of 97 kDa is in agreement with scientific literature (Moog-Lutz et al., J. Biol. Chem, Jun 2001; 276: 22375 - 22381).
Control
Jurkat Whole Cell Lysate
PRAM1 Overexpression Lysate
Publications
Read Publication using NB100-821.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PRAM1 Antibody

  • MGC39864
  • PML-RAR
  • PML-RARA regulated adaptor molecule 1
  • PML-RARA target gene encoding an Adaptor Molecule-1
  • PML-RARA-regulated adapter molecule 1
  • PRAM
  • PRAM-1

Background

PRAM-1 is an adaptor protein interacting with proteins involved in an ATRA-signaling pathway

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PRAM1 Antibody (NB100-821)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRAM1
Entrez
Uniprot