PRAM1 Antibody

Western Blot: PRAM1 Antibody [NB100-821] - Analysis of PRAM1 in Jurkat lysate using this antibody at 2 ug/ ml. Primary incubated for 12 hour. Detected using chemiluminescence.

• Reactivity: Hu, Po
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PRAM1 Antibody Summary

• Immunogen: Peptide with sequence DFCDPLENQPLPLGR corresponding to C-Terminus according to NP_115528.4.
• Predicted Species: Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRAM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1:100 - 1:2000
• Application Notes: WB: Approx. 90-100 kDa band observed in Jurkat lysates. Overnight incubation is recommended with this antibody. An 79 kDa band of PRAM-1 (predicted MW of 79 kDa band) and detected a migration with an apparent MW of 97 kDa is in agreement with scientific literature (Moog-Lutz et al., J. Biol. Chem, Jun 2001; 276: 22375 - 22381).
• Control:

  Jurkat Whole Cell Lysate
  PRAM1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PRAM1 Antibody

• MGC39864
• PML-RAR
• PML-RARA regulated adaptor molecule 1
• PML-RARA target gene encoding an Adaptor Molecule-1
• PML-RARA-regulated adapter molecule 1
• PRAM
• PRAM-1

Background

PRAM-1 is an adaptor protein interacting with proteins involved in an ATRA-signaling pathway

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRAM1 Antibody (NB100-821)(1)

Publication using NB100-821

• Moog-Lutz C, Peterson EJ, Lutz PG et al. PRAM-1 is a novel adaptor protein regulated by retinoic acid (RA) and promyelocytic leukemia (PML)-RA receptor alpha in acute promyelocytic leukemia cells. J Biol Chem 2001-06-22 [PMID: 11301322]

Bioinformatics

• Gene Symbol: PRAM1
• Entrez:
  • 84106(Human)
• Uniprot:
  • NP_115528.3(Human)