PRAM1 Overexpression Lysate (Native)


Western Blot: PRAM1 Overexpression Lysate (Adult Normal) [NBL1-14722] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PRAM1.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

PRAM1 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC206972 and based on accession number NM_032152. The protein contains a C-terminal DDK tag.
Homo sapiens PML-RARA regulated adaptor molecule 1 (PRAM1), mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
73.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for PRAM1

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PRAM1 Overexpression Lysate (Native)

  • MGC39864
  • PML-RARA regulated adaptor molecule 1
  • PML-RARA target gene encoding an Adaptor Molecule-1
  • PML-RARA-regulated adapter molecule 1
  • PRAM
  • PRAM-1


The protein encoded by this gene is similar to FYN binding protein (FYB/SLAP-130), an adaptor protein involved in T cell receptor mediated signaling. This gene is expressed and regulated during normal myelopoiesis. The expression of this gene is induced by retinoic acid and is inhibited by the expression of PML-RARalpha, a fusion protein of promyelocytic leukemia (PML) and the retinoic acid receptor-alpha (RARalpha). [provided by RefSeq].


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu
Applications: WB, IB, ICC/IF, IHC, IHC-P, IP, PLA
Species: Hu
Applications: WB, IHC
Species: Hu
Species: Hu
Applications: WB, Flow, IHC, IHC-P
Species: Hu
Applications: WB, Simple Western
Species: Hu, Mu
Applications: WB, ChIP, IP, PLA, CHIP-SEQ
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, PLA
Species: Hu
Applications: WB, Simple Western
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP, ChIP, ICC
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: IHC
Species: Mu
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB
Species: Hu
Applications: IP (-), WB
Species: Hu
Applications: WB

Publications for PRAM1 Lysate (NBL1-14722) (0)

There are no publications for PRAM1 Lysate (NBL1-14722).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PRAM1 Lysate (NBL1-14722) (0)

There are no reviews for PRAM1 Lysate (NBL1-14722). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PRAM1 Lysate (NBL1-14722) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional PRAM1 Products

Bioinformatics Tool for PRAM1 Lysate (NBL1-14722)

Discover related pathways, diseases and genes to PRAM1 Lysate (NBL1-14722). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PRAM1 Lysate (NBL1-14722)

Discover more about diseases related to PRAM1 Lysate (NBL1-14722).

Pathways for PRAM1 Lysate (NBL1-14722)

View related products by pathway.

PTMs for PRAM1 Lysate (NBL1-14722)

Learn more about PTMs related to PRAM1 Lysate (NBL1-14722).

Blogs on PRAM1

There are no specific blogs for PRAM1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

PRAM1 Antibody
CD11b Antibody

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our PRAM1 Overexpression Lysate (Native) and receive a gift card or discount.


Gene Symbol PRAM1