PPP2R5E Antibody

Western Blot: PPP2R5E Antibody [NB100-845] - Staining (0.1ug/ml) of Human Cerebellum (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PPP2R5E Antibody Summary

• Description: Novus Biologicals Goat PPP2R5E Antibody (NB100-845) is a polyclonal antibody validated for use in WB and ELISA. Anti-PPP2R5E Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-LKRGLRRDGIIPT corresponding to C-Terminus according to NP_006237.1.
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PPP2R5E
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1-0.3 ug/ml
• Application Notes: WB: Approx. 55 kDa band and 40 kDa band bands observed in mouse brain extracts. Please note that the two bands may correspond to two different isoforms. Two isoforms of the epsilon mRNA have been detected in Northern blots (J Biol Chem 1996 Sep 6;271(36): 22081-9). An anonymous customer has reported usage in human.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PPP2R5E Antibody

• epsilon isoform of regulatory subunit B56, protein phosphatase 2A
• PP2A B subunit isoform B56-epsilon
• PP2A B subunit isoform B'-epsilon
• PP2A B subunit isoform PR61-epsilon
• PP2A B subunit isoform R5-epsilon
• PP2A, B subunit, B' epsilon
• PP2A, B subunit, B56 epsilon
• PP2A, B subunit, PR61 epsilon
• PP2A, B subunit, R5 epsilon
• PPP2R5E
• protein phosphatase 2, regulatory subunit B (B56), epsilon isoform
• protein phosphatase 2, regulatory subunit B', epsilon isoform
• serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, epsilon
• serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilonisoform

Background

The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPP2R5E Antibody (NB100-845)(6)

Publications using NB100-845

• Santos A, Cristóbal I, Caramés C et al. Deregulation of the miR-19b/PPP2R5E Signaling Axis Shows High Functional Impact in Colorectal Cancer Cells International Journal of Molecular Sciences 2023-04-24 [PMID: 37175484] (Western Blot, Human)
• Cristobal I, Manso R, Rincon R et al. PP2A inhibition is a common event in colorectal cancer and its restoration using FTY720 shows promising therapeutic potential. Mol. Cancer Ther. 2014-01-22 [PMID: 24448818] (WB, Human)
• Cristobal I, Cirauqui C, Castello-Cros R et al. Downregulation of PPP2R5E is a common event in acute myeloid leukemia that affects the oncogenic potential of leukemic cells. Haematologica 2013-06-28 [PMID: 23812941] (WB, Human)
• Gao Q, Courtheoux T, Gachet Y et al. A non-ring-like form of the Dam1 complex modulates microtubule dynamics in fission yeast. Proc Natl Acad Sci U S A 2010-07-27 [PMID: 20624975]
• Mavrakis KJ, Wolfe AL, Oricchio E et al. Genome-wide RNA-mediated interference screen identifies miR-19 targets in Notch-induced T-cell acute lymphoblastic leukaemia. Nat Cell Biol. 2010-04-01 [PMID: 20190740] (WB, Mouse)
• McCright B, Virshup DM. Identification of a new family of protein phosphatase 2A regulatory subunits. J Biol Chem 1995-11-03 [PMID: 7592815]

Review for PPP2R5E Antibody (NB100-845) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 02/16/2009

Summary

• Application: Western Blot
• Sample Tested: HeLa cell, Sample Amount: 10ug
• Species: Human

Bioinformatics

• Gene Symbol: PPP2R5E
• Entrez:
  • 5529(Human)
  • 26932(Mouse)
• Uniprot:
  • Q16537(Human)