PPP1R9B/Spinophilin Antibody [Alexa Fluor® 488]

• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

PPP1R9B/Spinophilin Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human PPP1R9B
  Lys289-Asp412
  Accession # Q96SB3
• Specificity: Detects human and rat PPP1R9B in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

PPP1R9B (Protein phosphatase 1 regulatory subunit 9B; also spinophilin and neurabin-II) is a widely expressed intracellular protein that is found in both cytoplasm and nucleus. Although its predicted MW is 89 kDa, it runs anolamously at 120-140 kDa in SDS-Page. PPP1R9B is associated with actin polymerization, particularly in cells such as neurons and NK cells. In NK cells, it orchestrates actin organization around the immunological synapse, allowing for targeted release of cytotoxic granules. Human PPP1R9B is 815 amino acids (aa) in length. It contains one actin-binding region (aa 1-154), a PP1 binding domain (aa 415-492), a PZD domain (aa 493-583) and a coiled-coil region (aa 647-815). PPP1R9B is reported to form homotrimers and homotetramers. There is one isoform variant that shows an Ala insertion after both Ala158 and Ala165, coupled to a premature truncation after Arg576. Over aa 291-414, human PPP1R9B shares 91% aa identity with mouse PPP1R9B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.