PPAR gamma/NR1C3 Antibody

Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-76958] - Western blot analysis of PPAR gamma on PC-12 cell and human liver tissue lysate using anti-PPAR gamma antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: PPAR gamma/NR1C3 Antibody [NBP2-76958] - ICC staining PPAR gamma in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: PPAR gamma/NR1C3 Antibody [NBP2-76958] - Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-PPAR gamma antibody. Counter stained with hematoxylin.

Flow Cytometry: PPAR gamma/NR1C3 Antibody [NBP2-76958] - Flow cytometric analysis of A431 cells with PPAR gamma antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: PPAR gamma/NR1C3 Antibody [NBP2-76958] - ICC staining PPAR gamma in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: PPAR gamma/NR1C3 Antibody [NBP2-76958] - ICC staining PPAR gamma in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: PPAR gamma/NR1C3 Antibody [NBP2-76958] - Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PPAR gamma antibody. Counter stained with hematoxylin.

Immunohistochemistry: PPAR gamma/NR1C3 Antibody [NBP2-76958] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PPAR gamma antibody. Counter stained with hematoxylin.

Product Discontinued

PPAR gamma/NR1C3 Antibody Summary

• Immunogen: Recombinant protein within human PPAR gamma/NR1C3 aa 250-450. (SwissProt: P37231 Human)
• Localization: Nucleus. Cytoplasm.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPARG
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.5%BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for PPAR gamma/NR1C3 Antibody

• CIMT1
• NR1C3
• NR1C3GLM1
• Nuclear receptor subfamily 1 group C member 3
• peroxisome proliferator-activated receptor gamma 1
• peroxisome proliferator-activated receptor gamma
• PPAR gamma
• PPARG
• PPARG1peroxisome proliferative activated receptor, gamma
• PPARG2PPARgamma
• PPAR-gamma

Background

PPAR gamma(PPARG), which encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPAR gamma/NR1C3 Antibody (NBP2-76958)(1)

Publication using NBP2-76958

• Xu S, Lee E, Sun Z et al. Perilipin 2 Impacts Acute Kidney Injury via Regulation of PPAR alpha Journal of immunology research 2021-09-09 [PMID: 34541006] (WB, Mouse)

Bioinformatics

• Gene Symbol: PPARG