![PPAR delta/NR1C2 Assay Kit (Colorimetric) [KA1359] - Increasing amounts of positive control (total lysate) are assayed for PPARD DNA-binding activity.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Assay-Kit-Colorimetric-KA1359-img0001.jpg "PPAR delta/NR1C2 Assay Kit (Colorimetric) [KA1359] - Increasing amounts of positive control (total lysate) are assayed for PPARD DNA-binding activity.")
| Applications | Func |
| Suitable Sample Type | Nuclear Extracts |
| Description | PPAR delta/NR1C2 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting PPAR delta/NR1C2 transcription factor DNA binding activity in nuclear extracts. |
| Assay Type | Colorimetric |
| Sample Volume | 10 uL/well. All samples should be assayed in duplicate at minimum. |
| Kit Type | Transcription Factor Assay Kit (Colorimetric) |
| Gene | PPARD |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Components |
|
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![Western Blot: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - Affinity Purified PPAR delta/NR1C2/NR1C2 antibody is shown to detect a predominant band at ~ 40kDa corresponding to PPAR delta/NR1C2 present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30ug of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4 C followed reaction with a 1:10,000 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green). IRDye(R) 800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://images.novusbio.com/images/PPAR-delta-NR1C2-Antibody---N-terminal-Western-Blot-NB600-637-img0007.jpg "Western Blot: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - Affinity Purified PPAR delta/NR1C2/NR1C2 antibody is shown to detect a predominant band at ~ 40kDa corresponding to PPAR delta/NR1C2 present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30ug of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4 C followed reaction with a 1:10,000 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green). IRDye(R) 800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")