PPAR delta/NR1C2 Antibody

Immunohistochemistry-Paraffin: PPAR delta/NR1C2 Antibody [NBP1-03289] - Staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Discontinued

PPAR delta/NR1C2 Antibody Summary

• Immunogen: Synthetic peptide corresponding to aa near the amino terminus of mouse PPAR delta/NR1C2 aa 1-14.
• Specificity: No reactivity is expected against other subtypes of PPAR.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPARD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:30000-1:70000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1:500-1:5000
• Application Notes: IHC: NBP1-03289 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PPAR delta/NR1C2 Antibody

• FAAR
• MGC3931
• NR1C2
• NR1C2PPAR-delta
• NUC1
• NUC1PPAR-beta
• NUCI
• NUCII
• Nuclear hormone receptor 1
• Nuclear receptor subfamily 1 group C member 2
• peroxisome proliferative activated receptor, delta
• Peroxisome proliferator-activated receptor beta
• peroxisome proliferator-activated receptor delta
• PPAR delta
• PPARB
• PPARD

Background

Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPARD
• Entrez:
  • 5467(Human)
  • 19015(Mouse)
• Uniprot:
  • Q03181(Human)