PPAR delta/NR1C2 Antibody (4E3-1B11) - Azide and BSA Free

Western Blot: PPAR delta/NR1C2 Antibody (4E3-1B11) [H00005467-M01] - PPARD monoclonal antibody (M01), clone 4E3-1B11 Analysis of PPARD expression in Jurkat.

Proximity Ligation Assay: PPAR delta/NR1C2 Antibody (4E3-1B11) [H00005467-M01] - Analysis of protein-protein interactions between HDAC2 and PPARD. HeLa cells were stained with anti-HDAC2 rabbit purified polyclonal 1:1200 and anti-PPARD mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: PPAR delta/NR1C2 Antibody (4E3-1B11) [H00005467-M01] - Detection limit for recombinant GST tagged PPARD is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Func
• Clone: 4E3-1B11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PPAR delta/NR1C2 Antibody (4E3-1B11) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PPARD (AAH02715, 1 a.a. ~ 361 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEQPQEEAPEVREEEEKEEVAEAEGAPELNGGPQHALPSSSYTDLSRSSSPPSLLDQLQMGCDGASCGSLNMECRVCGDKASGFHYGVHACEGCKGFFRRTIRMKLEYEKCERSCKIQKKNRNKCQYCRFQKCLALGMSHNAIRFGRMPEAEKRKLVAGLTANEGSQYNPQVADLKAFSKHIYNAYLKNFNMTKKKARSILTGKASHTAPFVIHDIETLWQAEKGLVWKQLVNGLPPYKEISVHVFYRCQCTTVETVRELTEFAKSIPSFSSLFLNDQVTLLKYGVHEAIFAMLASIVNKDGLLVANGSGFVTREFLRSLRKPFSDIIEPKFEFAVKFNALELDDSDLALFIAAIILCGGE
• Specificity: PPARD - peroxisome proliferative activated receptor, delta
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPARD
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PPAR delta/NR1C2 Antibody (4E3-1B11) - Azide and BSA Free

• FAAR
• MGC3931
• NR1C2
• NR1C2PPAR-delta
• NUC1
• NUC1PPAR-beta
• NUCI
• NUCII
• Nuclear hormone receptor 1
• Nuclear receptor subfamily 1 group C member 2
• peroxisome proliferative activated receptor, delta
• Peroxisome proliferator-activated receptor beta
• peroxisome proliferator-activated receptor delta
• PPAR delta
• PPARB
• PPARD

Background

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPAR delta/NR1C2 Antibody (H00005467-M01)(1)

Publication using H00005467-M01

• Holloway GP, Perry CG, Thrush AB et al. PGC1{alpha} relationship with skeletal muscle palmitate oxidation is not present with obesity, despite maintained ained PGC1{alpha} and PGC1{beta} protein. Am J Physiol Endocrinol Metab 2008-03-18 [PMID: 18349111]

Bioinformatics

• Gene Symbol: PPARD
• Entrez:
  • 5467(Human)
• OMIM:
  • 600409(Human)
• Uniprot:
  • AAH02715(Human)