POU3F3 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: POU3F3 Antibody [NBP2-57011] - Staining of human cell line SH-SY5Y shows localization to nucleus. Antibody staining is shown in green.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

POU3F3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit POU3F3 Antibody - BSA Free (NBP2-57011) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-POU3F3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: GGAQSLVHPGLVRGDTPELAEHHHHH
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POU3F3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry-Frozen Reported in scientific literature (PMID:33649223)
• Application Notes: ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  POU3F3 Recombinant Protein Antigen (NBP2-57011PEP)

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33649223).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for POU3F3 Antibody - BSA Free

• brain-1
• Brain-specific homeobox/POU domain protein 1
• Brn-1
• BRN1brn-1
• Oct-8
• Octamer-binding protein 8
• Octamer-binding transcription factor 8
• OTF-8
• OTF8oct-8
• POU class 3 homeobox 3
• POU domain class 3, transcription factor 3
• POU domain, class 3, transcription factor 3

Background

POU3F3, also known as POU Class 3 Homeobox 3, is a transcription factor that is primarily expressed in the brain and CNS. POU3F3 interacts with SOX4 and SOX11, and is implicated in the development of neurons. Current research surrounding POU3F3 has shown potential linkages with neuronitis and cerebritis. POU3F3 has also been shown to interact with POU3F4, CAV1, SOX10, and GADD34.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for POU3F3 Antibody (NBP2-57011)(2)

Publications using NBP2-57011

• Tsujimoto, H;Hoshina, A;Mae, SI;Araoka, T;Changting, W;Ijiri, Y;Nakajima-Koyama, M;Sakurai, S;Okita, K;Mizuta, K;Niwa, A;Saito, MK;Saitou, M;Yamamoto, T;Graneli, C;Woollard, KJ;Osafune, K; Selective induction of human renal interstitial progenitor-like cell lineages from iPSCs reveals development of mesangial and EPO-producing cells Cell reports 2023-12-22 [PMID: 38237600]
• Ruan X, Kang B, Qi C et al. Progenitor cell diversity in the developing mouse neocortex Proceedings of the National Academy of Sciences of the United States of America 2021-03-09 [PMID: 33649223] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: POU3F3