POR/Cytochrome P450 Reductase Antibody - BSA Free

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Analysis in human liver and skeletal muscle tissues. Corresponding POR RNA-seq data are presented for the same tissues.

Western Blot: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Analysis in human cell line SK-BR-3.

Immunocytochemistry/Immunofluorescence: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Staining of human cell line A-431 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes as expected.

Western Blot: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP1-85357] - Staining of human liver shows very strong granular cytoplasmic positivity in hepatocytes.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

POR/Cytochrome P450 Reductase Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit POR/Cytochrome P450 Reductase Antibody - BSA Free (NBP1-85357) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: KEEVPEFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKFAVFGLGNKTYEHFNA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POR
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  POR/Cytochrome P450 Reductase Protein (NBP1-85357PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for POR/Cytochrome P450 Reductase Antibody - BSA Free

• CPR
• CYPOR
• CYPORFLJ26468
• Cytochrome P450 Reductase
• DKFZp686G04235
• EC 1.6.2.4
• NADPH--cytochrome P450 reductase
• NADPH-dependent cytochrome P450 reductase
• P450 (cytochrome) oxidoreductase
• P450R
• POR

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POR