POR/Cytochrome P450 Reductase Antibody

Images

 
Western Blot: POR/Cytochrome P450 Reductase Antibody [NBP1-32364] - A. 50 ug mouse lung lysate/extract 7.5% SDS-PAGE POR/Cytochrome P450 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to ...read more
Immunocytochemistry/ Immunofluorescence: POR/Cytochrome P450 Reductase Antibody [NBP1-32364] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP1-32364] - Sample: Paraffin-embedded ovarian carcinoma. POR/Cytochrome P450 antibody dilution: 1:500.
Western Blot: POR/Cytochrome P450 Reductase Antibody [NBP1-32364] - Wild-type (WT) and POR knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with POR/Cytochrome P450 ...read more

Product Details

Summary
Reactivity Hu, Rt, Po, Bv, Ca, GP, Rb, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.45 mg/ml
     

Genetic Strategies

   

Order Details

POR/Cytochrome P450 Reductase Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human POR. The exact sequence is proprietary.
Localization
Endoplasmic reticulum membrane, Peripheral membrane protein
Predicted Species
Rat (92%), Porcine (92%), Rabbit (93%), Rhesus Macaque (99%), Canine (91%), Guinea Pig (91%), Bovine (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Chicken (85%), Xenopus laevis (85%),

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.45 mg/ml
Purity
Immunogen affinity purified

Alternate Names for POR/Cytochrome P450 Reductase Antibody

  • CPR
  • CYPOR
  • CYPORFLJ26468
  • Cytochrome P450 Reductase
  • DKFZp686G04235
  • EC 1.6.2.4
  • NADPH--cytochrome P450 reductase
  • NADPH-dependent cytochrome P450 reductase
  • P450 (cytochrome) oxidoreductase
  • P450R
  • POR

Background

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for POR/Cytochrome P450 Reductase Antibody (NBP1-32364)

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Diseases for POR/Cytochrome P450 Reductase Antibody (NBP1-32364)

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Bioinformatics

Gene Symbol POR
Entrez