PON1 Antibody (453223)

Western blot shows lysates of mouse liver tissue, human liver tissue and an enriched HDL/LDL fraction of human plasma obtained from ultracentrifugation. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse PON1 Monoclonal Antibody (Catalog # MAB4926) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PON1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 453223
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

PON1 Antibody (453223) Summary

• Immunogen: E. coli-derived recombinant human PON1
  Ala30-Leu355
  Accession # P27169
• Specificity: Detects endogenous human and mouse PON1 in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) PON2 or rhPON3.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: PON1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PON1 Antibody (453223)

• A-esterase 1
• Aromatic esterase 1
• arylesterase B-type
• EC 3.1.1.2
• EC 3.1.1.81
• EC 3.1.8.1
• ESA
• ESAesterase A
• K-45
• MVCD5
• paraoxonase 1
• PON 1
• PON1
• PONparaoxonase B-type
• serum aryldiakylphosphatase
• Serum aryldialkylphosphatase 1
• serum paraoxonase/arylesterase 1

Background

The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7. Paraoxonase/arylesterase 1 (PON1, also known as serum paraoxonase) is a 355 amino acid, 43 kDa glycoprotein that is expressed in liver and is secreted into the bloodstream where it associates with high-density lipoproteins (HDL). Serum PON1 concentrations vary widely among normal individuals, in part due to differential expression of some polymorphisms. Sequence polymorphisms in this gene may be associated with coronary heart disease and a number of phenotypes related to diabetes. PON1 is primarily a lactonase (EC 3.1.8.1) that is thought to attenuate the oxidation of low-density lipoproteins (LDL). This may slow the initiation and progression of atherosclerosis. Human PON1 shares 83% and 81% aa identity with mouse and rat PON1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PON1 Antibody (MAB4926)(2)

Publications using MAB4926

• Dawoud Sulaiman, Leo Sungwong Choi, Hyeong Min Lee, Jaejin Shin, Dong Hwan Kim, Keun Woo Lee et al. Vutiglabridin Modulates Paraoxonase 1 and Ameliorates Diet-Induced Obesity in Hyperlipidemic Mice Biomolecules 2023-04-18 [PMID: 37189434]
• Dawoud Sulaiman, Leo Sungwong Choi, Hyeong Min Lee, Jaejin Shin, Dong Hwan Kim, Keun Woo Lee et al. Vutiglabridin Modulates Paraoxonase 1 and Ameliorates Diet-Induced Obesity in Hyperlipidemic Mice Biomolecules 2023-04-18 [PMID: 37189434] (Western Blot, Mouse, Transgenic Mouse)

Bioinformatics

• Gene Symbol: PON1
• Uniprot:
  • P27169(Human)