POLR2G Antibody - BSA Free

Western Blot: POLR2G Antibody [NBP2-95215] - Analysis of extracts of various cell lines, using POLR2G at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

POLR2G Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit POLR2G Antibody - BSA Free (NBP2-95215) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human POLR2G (NP_002687.1). MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFDPNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDDYLGLVS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLR2G
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for POLR2G Antibody - BSA Free

• DNA directed RNA polymerase II 19 kda polypeptide
• DNA-directed RNA polymerase II 19 kDa polypeptide
• DNA-directed RNA polymerase II subunit G
• DNA-directed RNA polymerase II subunit RPB7
• hRPB19
• hsRPB7
• MGC138369
• polymerase (RNA) II (DNA directed) polypeptide G
• RNA polymerase II 19 kDa subunit
• RNA polymerase II seventh subunit
• RNA polymerase II subunit B7
• RPB19
• RPB7MGC138367

Background

POLR2G encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, the association of this subunit with the polymerase under suboptimal growth conditions indicates it may play a role in regulating polymerase function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR2G