POLR2E Antibody (S05-8J5)

Western Blot: POLR2E Antibody (S05-8J5) [NBP3-14993] - Western blot detection of POLR2E in K562, C6, 3T3, Hela cell lysates using POLR2E Rabbit mAb (1:1000 diluted). Predicted band size: 25kDa. Observed band size: 25kDa.

Immunohistochemistry-Paraffin: POLR2E Antibody (S05-8J5) [NBP3-14993] - Immunohistochemistry of POLR2E in paraffin-embedded Human breast cancer tissue using POLR2E Rabbit mAb at dilution 1/20

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: S05-8J5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

POLR2E Antibody (S05-8J5) Summary

• Description: Novus Biologicals Rabbit POLR2E Antibody (S05-8J5) (NBP3-14993) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human POLR2E (Uniprot#: P19388)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: POLR2E
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/20
  • Immunohistochemistry-Paraffin
  • Western Blot 1/2000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for POLR2E Antibody (S05-8J5)

• DNA directed RNA polymerase II 23 kda polypeptide
• DNA-directed RNA polymerase II 23 kDa polypeptide
• DNA-directed RNA polymerase II subunit E
• DNA-directed RNA polymerases I, II, and III subunit RPABC1
• hRPB25
• hsRPB5
• polymerase (RNA) II (DNA directed) polypeptide E (25kD)
• polymerase (RNA) II (DNA directed) polypeptide E, 25kDa
• RPABC1
• RPB5 homolog
• RPB5
• XAP4RNA polymerases I, II, and III subunit ABC1

Background

POLR2E encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR2E