POLR2D Overexpression Lysate (Native)

Images

 
Western Blot: POLR2D Overexpression Lysate (Adult Normal) [NBL1-14581] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for POLR2D.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

POLR2D Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201116 and based on accession number NM_004805. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens polymerase (RNA) II (DNA directed) polypeptide D (POLR2D), mRNA.
Gene
POLR2D

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
16.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for POLR2D

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for POLR2D Overexpression Lysate (Native)

  • DNA-directed RNA polymerase II 16 kDa polypeptide
  • DNA-directed RNA polymerase II subunit D
  • DNA-directed RNA polymerase II subunit RPB4
  • HSRBP4
  • HSRPB4
  • polymerase (RNA) II (DNA directed) polypeptide D
  • RBP4
  • RNA polymerase II 16 kDa subunit
  • RNA polymerase II subunit B4
  • RNA polymerase II subunit hsRBP4
  • RPB16

Background

This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP1-89649
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-594
Species: Hu, Mu, Ca
Applications: WB, IHC-P
NBP1-49533
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-80851
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-88000
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF1857
Species: Mu
Applications: WB, Simple Western, IP
AF1443
Species: Mu, Rt
Applications: WB, Simple Western, IHC, ICC
NBP2-22106
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC-P
NBL1-14581
Species: Hu
Applications: WB

Publications for POLR2D Lysate (NBL1-14581) (0)

There are no publications for POLR2D Lysate (NBL1-14581).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for POLR2D Lysate (NBL1-14581) (0)

There are no reviews for POLR2D Lysate (NBL1-14581). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for POLR2D Lysate (NBL1-14581) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional POLR2D Products

Bioinformatics Tool for POLR2D Lysate (NBL1-14581)

Discover related pathways, diseases and genes to POLR2D Lysate (NBL1-14581). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for POLR2D Lysate (NBL1-14581)

Discover more about diseases related to POLR2D Lysate (NBL1-14581).
 

Pathways for POLR2D Lysate (NBL1-14581)

View related products by pathway.

Research Areas for POLR2D Lysate (NBL1-14581)

Find related products by research area.

Blogs on POLR2D

There are no specific blogs for POLR2D, but you can read our latest blog posts.

Customers Who Bought This Also Bought

POLR2D Antibody
NBP1-92280

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our POLR2D Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol POLR2D