POLR2D Overexpression Lysate (Native)


Western Blot: POLR2D Overexpression Lysate (Adult Normal) [NBL1-14581] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for POLR2D.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

POLR2D Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC201116 and based on accession number NM_004805. The protein contains a C-terminal DDK tag.
Homo sapiens polymerase (RNA) II (DNA directed) polypeptide D (POLR2D), mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
16.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for POLR2D

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for POLR2D Overexpression Lysate (Native)

  • DNA-directed RNA polymerase II 16 kDa polypeptide
  • DNA-directed RNA polymerase II subunit D
  • DNA-directed RNA polymerase II subunit RPB4
  • HSRBP4
  • HSRPB4
  • polymerase (RNA) II (DNA directed) polypeptide D
  • RBP4
  • RNA polymerase II 16 kDa subunit
  • RNA polymerase II subunit B4
  • RNA polymerase II subunit hsRBP4
  • RPB16


This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Gene Symbol POLR2D