Podoplanin Antibody (8.1.1) - BSA Free

Western Blot: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Podoplanin is differentially expressed in primary podocytes relative to primary PECs after six passages in culture (arrow), consistent with mRNA expression analysis. Lysates of isolated glomeruli are used as positive control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22529955) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- at 5 ug/ml overnight at 4C and detected with an anti-Golden Syrian hamster DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Staining for podoplanin (red) and cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous mesothelioma. In A, the lining mesothelium (solid arrows) stained positively for podoplanin in this double label immunofluorescent image of diaphragm. The cells beneath the mesothelial lining are also red due to expression of podoplanin and these are cells of a malignant serosal tumor. Promotion of lung adenocarcinoma following inhalation exposure to multi-walled carbon nanotubes. Part Fibre Toxicol (2014)

Western Blot: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Analysis of Podoplanin in mouse kidney tissue extract.

Immunocytochemistry/Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Macrophages were isolated from wild-type (WT) mice treated with acetaminophen (APAP) for 3 hr. The cells were treated in vitro with either control IgG (Ctrl IgG) or an anti-podoplanin antibody (alpha-podoplanin Ab) before incubation with platelets. Immunofluorescence (IF) staining was performed to detect podoplanin on Macrophages and C-type lectin-like receptor 2 (Clec-2) on platelets. Scale bar, 25 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34110284/) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Analysis of Podoplanin in mouse mammary gland after MDA-MB-231 orthotopic transplantation. Image courtesy of product review by Luana Schito.

Immunohistochemistry: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Podoplanin (8.1.1) antibody labeling (Green) of glomeruli from mouse kidney. Nuclei were counterstained with DAPI (Blue).

Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Staining for podoplanin (red) and cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous mesothelioma. In C, a double label immunofluorescent image demonstrates a malignant serosal tumor between the liver and gall bladder that is lined by reactive mesothelium (solid arrow) which stains red for podoplanin as well as green for cytokeratins. The cells of the subjacent malignant serosal tumor stain weakly red for podoplanin. Promotion of lung adenocarcinoma following inhalation exposure to multi-walled carbon nanotubes. Part Fibre Toxicol (2014)

Podolanin expressing on Mɸs mediates interactions with platelets.Mɸs were isolated from wild-type (WT) mice treated with acetaminophen (APAP) for 3 hr. The cells were treated in vitro with either control IgG (Ctrl IgG) or an anti-podoplanin antibody ( alpha -podoplanin Ab) before incubation with platelets. Immunofluorescence (IF) staining was performed to detect podoplanin on Mɸs and C-type lectin-like receptor 2 (Clec-2) on platelets. Scale bar, 25 μm.

Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Immunofluorescence staining for podoplanin (red) & cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous mesothelioma. In A, the lining mesothelium (solid arrows) stained positively for podoplanin in this double label immunofluorescent image of diaphragm. The cells beneath the mesothelial lining are also red due to expression of podoplanin & these are cells of a malignant serosal tumor. In B, only the single label green fluorescence is shown to demonstrate weak expression of cytokeratins in the lining mesothelium (solid arrow), while staining of the subjacent tumor for cytokeratins is equivocal. In C, a double label immunofluorescent image demonstrates a malignant serosal tumor between the liver & gall bladder that is lined by reactive mesothelium (solid arrow) which stains red for podoplanin as well as green for cytokeratins. The cells of the subjacent malignant serosal tumor stain weakly red for podoplanin. In D, the photomicrograph shows this same tumor but only the green fluorescence for cytokeratins. The reactive mesothelium lining the malignant serosal tumor (solid arrow) stains green for cytokeratins while the serosal tumor has no evidence of cytokeratin expression. The normal mesothelium lining the liver is weakly positive for cytokeratins (dashed arrow). The epithelium lining the gall bladder (open arrow) strongly expresses cytokeratins. Magnification bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-11-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Immunofluorescence staining for podoplanin (red) & cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous mesothelioma. In A, the lining mesothelium (solid arrows) stained positively for podoplanin in this double label immunofluorescent image of diaphragm. The cells beneath the mesothelial lining are also red due to expression of podoplanin & these are cells of a malignant serosal tumor. In B, only the single label green fluorescence is shown to demonstrate weak expression of cytokeratins in the lining mesothelium (solid arrow), while staining of the subjacent tumor for cytokeratins is equivocal. In C, a double label immunofluorescent image demonstrates a malignant serosal tumor between the liver & gall bladder that is lined by reactive mesothelium (solid arrow) which stains red for podoplanin as well as green for cytokeratins. The cells of the subjacent malignant serosal tumor stain weakly red for podoplanin. In D, the photomicrograph shows this same tumor but only the green fluorescence for cytokeratins. The reactive mesothelium lining the malignant serosal tumor (solid arrow) stains green for cytokeratins while the serosal tumor has no evidence of cytokeratin expression. The normal mesothelium lining the liver is weakly positive for cytokeratins (dashed arrow). The epithelium lining the gall bladder (open arrow) strongly expresses cytokeratins. Magnification bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-11-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Immunofluorescence staining for podoplanin (red) & cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous mesothelioma. In A, the lining mesothelium (solid arrows) stained positively for podoplanin in this double label immunofluorescent image of diaphragm. The cells beneath the mesothelial lining are also red due to expression of podoplanin & these are cells of a malignant serosal tumor. In B, only the single label green fluorescence is shown to demonstrate weak expression of cytokeratins in the lining mesothelium (solid arrow), while staining of the subjacent tumor for cytokeratins is equivocal. In C, a double label immunofluorescent image demonstrates a malignant serosal tumor between the liver & gall bladder that is lined by reactive mesothelium (solid arrow) which stains red for podoplanin as well as green for cytokeratins. The cells of the subjacent malignant serosal tumor stain weakly red for podoplanin. In D, the photomicrograph shows this same tumor but only the green fluorescence for cytokeratins. The reactive mesothelium lining the malignant serosal tumor (solid arrow) stains green for cytokeratins while the serosal tumor has no evidence of cytokeratin expression. The normal mesothelium lining the liver is weakly positive for cytokeratins (dashed arrow). The epithelium lining the gall bladder (open arrow) strongly expresses cytokeratins. Magnification bar is 50 μm. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-11-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Mu, Hu(-)
• Applications: WB, Flow, ICC/IF, IHC, IP, MS, CyTOF-ready
• Clone: 8.1.1
• Clonality: Monoclonal
• Host: Golden Syrian Hamster
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Podoplanin Antibody (8.1.1) - BSA Free Summary

• Background: Podoplanin is a mucine-type glycoprotein which induces platelet aggregation. Podoplanin has been implicated in cancer cell migration, invasion, metastasis, and malignant progression. Podoplanin antibodies serve as markers of lymphatic endothelium and fibroblastic reticular cells of lymphoid organs, as well as certain lung injuries. The upregulation of podoplanin in tumor cells has been cor...related with an increase in metastasis and motility. Recent research has shown that Podoplanin plays a critical role in immune cells, including T cells and dendritic cells.
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• Immunogen: Murine thymic stromal cell lines
• Localization: Membrane; Single-pass type I membrane protein.
• Marker: Lymphatic Endothelium Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Golden Syrian Hamster
• Gene: PDPN
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Electron Microscopy
  • Flow Cytometry 1:400
  • Immunocytochemistry/ Immunofluorescence 1-5ug/ml
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000-1:2000
• Application Notes: Optimal dilutions/concentrations should be determined by the end user. This antibody is CyTOF ready.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Does not cross-react with human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Podoplanin Antibody (8.1.1) - BSA Free

• 36-KD
• Aggrus
• Gp36
• Gp38
• GP40
• HT1A-1
• hT1alpha-1
• hT1alpha-2
• lung type I cell membrane associated glycoprotein
• lung type-I cell membrane-associated glycoprotein (T1A-2)
• OTS 8
• OTS8
• PA2.26 antigen
• PDPN
• Podoplanin
• RANDAM-2
• T1A
• T1A-2
• T1-alpha

Background

Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a 38 kDa membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Podoplanin Antibody (NB600-1015)(23)

Publications using NB600-1015

• Xia CH, Lin W, Li R et al. Altered Cell Clusters and Upregulated Aqp1 in Connexin 50 Knockout Lens Epithelium Investigative Ophthalmology & Visual Science 2024-09-17 [PMID: 39287589]
• Guo L, Bao W, Yang S et al. Rhei Radix et Rhizoma in Xuanbai-Chengqi decoction strengthens the intestinal barrier function and promotes lung barrier repair in preventing severe viral pneumonia induced by influenza A virus Journal of ethnopharmacology 2023-09-30 [PMID: 37783404] (IHC-P, Mouse)
  
  Details:
  1:100 dilution
• Tang AT, Buchholz DW, Szigety KM et al. Cell-autonomous requirement for ACE2 across organs in lethal mouse SARS-CoV-2 infection PLoS biology 2023-02-01 [PMID: 36745682] (IHC, Mouse)
• Fraser K, Hubbs A, Yanamala N et al. Histopathology of the broad class of carbon nanotubes and nanofibers used or produced in U.S. facilities in a murine model Particle and fibre toxicology 2021-12-20 [PMID: 34923995] (IHC-P, Mouse)
• S Roy, S Kumaravel, P Banerjee, TK White, A O'Brien, C Seelig, R Chauhan, B Ekser, KJ Bayless, G Alpini, SS Glaser, S Chakrabort Tumor Lymphatic Interactions Induce CXCR2-CXCL5 Axis and Alter Cellular Metabolism and Lymphangiogenic Pathways to Promote Cholangiocarcinoma Cells, 2021-11-09;10(11):. 2021-11-09 [PMID: 34831316]
• Shan Z, Li L, Atkins CL et al. Chitinase 3-like-1 contributes to acetaminophen-induced liver injury by promoting hepatic platelet recruitment eLife 2021-06-10 [PMID: 34110284] (IHC-P, Mouse)
• Neto LMM, Zufelato N, de Sousa-Junior AA et al. Specific T cell induction using iron oxide based nanoparticles as subunit vaccine adjuvant Hum Vaccin Immunother 2018-06-18 [PMID: 29913109] (Mouse)
• Asfahani RI, Tahoun MM, Miller-Hodges EV et al. Activation of podocyte Notch mediates early Wt1 glomerulopathy. kidney Int. 2018-04-01 [PMID: 29398135] (ICC/IF, Mouse)
• Jia Y, Chen K, Lin P et al. Treatment of acute lung injury by targeting MG53-mediated cell membrane repair. Nat Commun 2014-07-18 [PMID: 25034454] (IHC-P, Mouse)
  
  Details:
  Podoplanin/AT1 alpha antibody used in IHC-P as a specific cell marker for type I alveolar epithelial cells in lungs of wild-type and mg53-/- mice - tissue fixed in 4% neutral-buffered paraformaldehyde for 24 hous at 4C, paraffin blocks cut into 4um sections, staining detection with IF labelled secondary (Figure 1e and 1f; Supplementary Figure 2).
• Sargent LM, Porter DW, Staska LM et al. Promotion of lung adenocarcinoma following inhalation exposure to multi-walled carbon nanotubes. Part Fibre Toxicol 2014-01-15 [PMID: 24405760] (IHC-P, Mouse)
• Kabgani N, Grigoleit T, Schulte K, Sechi A, Sauer-Lehnen S, Tag C, Boor P, Kuppe C, Warsow G, Schordan S, Mostertz J, Chilukoti RK, Homuth G, Endlich N, Tacke F, Weiskirchen R, Fuellen G, Endlich K, Floege J, Smeets B, Moeller MJ. Primary cultures of glomerular parietal epithelial cells or podocytes with proven origin. PLoS One;7(4). 2012-01-01 [PMID: 22529955] (WB, Mouse)
• Porter DW, Hubbs AF, Mercer RR et al. Mouse pulmonary dose- and time course-responses induced by exposure to multi-walled carbon nanotubes. Toxicology 2010-03-10 [PMID: 19857541] (ICC/IF, IF/IHC, Mouse)
• Mahtab EA, Vicente-Steijn R, Hahurij ND, Jongbloed MR, Wisse LJ, DeRuiter MC, Uhrin P, Zaujec J, Binder BR, Schalij MJ, Poelmann RE, Gittenberger-de Groot AC. Podoplanin deficient mice show a RhoA-related hypoplasia of the sinus venosus myocardium including the sinoatrial node. Dev Dyn238(1):183-93. 2009-01-01 [PMID: 19097191]
• Mahtab EA, Wijffels MC, Van Den Akker NM, Hahurij ND, Lie-Venema H, Wisse LJ, Deruiter MC, Uhrin P, Zaujec J, Binder BR, Schalij MJ, Poelmann RE, Gittenberger-De Groot AC. Cardiac malformations and myocardial abnormalities in podoplanin knockout mouse embryos: Correlation with abnormal epicardial development. Dev Dyn237(3):847-57. 2008-03-01 [PMID: 18265012]
• Withers DR, Kim MY, Bekiaris V, Rossi SW, Jenkinson WE, Gaspal F, McConnell F, Caamano JH, Anderson G, Lane PJ. The role of lymphoid tissue inducer cells in splenic white pulp development. Eur J Immunol37(11):3240-5. 2007-11-01 [PMID: 17948268]
• Gittenberger-de Groot AC, Mahtab EA, Hahurij ND, Wisse LJ, Deruiter MC, Wijffels MC, Poelmann RE. Nkx2.5-negative myocardium of the posterior heart field and its correlation with podoplanin expression in cells from the developing cardiac pacemaking and conduction system. Anat Rec. 2007-01-01 [PMID: 17441204]
• Hara T, Katakai T, Lee JH, Nambu Y, Nakajima-Nagata N, Gonda H, Sugai M, Shimizu A. A transmembrane chemokine, CXC chemokine ligand 16, expressed by lymph node fibroblastic reticular cells has the potential to regulate T cell migration and adhesion. Int Immunol;18(2):301-11. 2006-02-01 [PMID: 16410312]
• Schacht V, Dadras SS, Johnson LA et al. Up-regulation of the lymphatic marker podoplanin, a mucin-type transmembrane glycoprotein, in human squamous cell carcinomas and germ cell tumors. Am J Pathol. 2005-03-01 [PMID: 15743802] (IHC-P, Mouse)
  
  Details:
  IHC (paraffin), Figs. 3A-H, 4A-L, 5A-J.
• Crnic I, Strittmatter K, Cavallaro U, Kopfstein L, Jussila L, Alitalo K, Christofori G. Loss of neural cell adhesion molecule induces tumor metastasis by up-regulating lymphangiogenesis. Cancer Res;64(23):8630-8. 2004-12-01 [PMID: 15574770] (IHC-P, Mouse)
• Katakai T, Hara T, Sugai M et al. Lymph node fibroblastic reticular cells construct the stromal reticulum via contact with lymphocytes. J Exp Med;200(6):783-95. 2004-09-20 [PMID: 15381731] (FLOW, ICC/IF, IHC-Fr, Mouse)
• Schacht V, Ramirez MI, Hong YK et al. T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema. EMBO J;22(14):3546-56. 2003-07-15 [PMID: 12853470] (EM, IHC-P, Mouse)
• Farr AG, Berry ML, Kim A, Nelson AJ, Welch MP, Aruffo A. Characterization and cloning of a novel glycoprotein expressed by stromal cells in T-dependent areas of peripheral lymphoid tissues. J Exp Med;176(5):1477-82. 1992-11-01 [PMID: 1402691] (WB, IHC-Fr, Mouse)
• Farr A, Nelson A, Hosier S. Characterization of an antigenic determinant preferentially expressed by type I epithelial cells in the murine thymus. J Histochem Cytochem;40(5):651-64. 1992-05-01 [PMID: 1374092]

Review for Podoplanin Antibody (NB600-1015) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-P Mouse 05/17/2011

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse mammary gland after MDA-MB-231 orthotopic transplantation
• Species: Mouse
• Lot: A1

Bioinformatics

• Gene Symbol: PDPN
• Entrez:
  • 14726(Mouse)
• Uniprot:
  • Q62011(Mouse)