Podoplanin Antibody (8.1.1) - BSA Free

Images

 
Western Blot: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Podoplanin is differentially expressed in primary podocytes relative to primary PECs after six passages in culture (arrow), consistent with mRNA ...read more
Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton ...read more
Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Staining for podoplanin (red) and cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous ...read more
Western Blot: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Analysis of Podoplanin in mouse kidney tissue extract.
Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Macrophages were isolated from wild-type (WT) mice treated with acetaminophen (APAP) for 3 hr. The cells were treated in ...read more
Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Analysis of Podoplanin in mouse mammary gland after MDA-MB-231 orthotopic transplantation. Image courtesy of product review by Luana ...read more
Immunohistochemistry: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Podoplanin (8.1.1) antibody labeling (Green) of glomeruli from mouse kidney. Nuclei were counterstained with DAPI (Blue).
Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) - BSA Free [NB600-1015] - Staining for podoplanin (red) and cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous ...read more
Podolanin expressing on Mɸs mediates interactions with platelets.Mɸs were isolated from wild-type (WT) mice treated with acetaminophen (APAP) for 3 hr. The cells were treated in vitro with either control IgG (Ctrl ...read more
Characterization of the primary PEC lines.PECs were negative for the endothelial marker von Willebrand factor (vWT, A) and for myofibroblast marker alpha-SMA (B). HUVECs (A') or human dermal fibroblasts (B') were used ...read more
SMC2 (a) and SMC4 (b) in HaCaT keratinocytes treated with control shRNA (upper panels) and treated with Nesprin-2-SMC domain specific shRNA (lower panels). Nesprin-2 was detected with mAb K20-478. Bar, 10 μm. (c) ...read more

Product Details

Summary
Reactivity Mu, Hu(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IP, MS, CyTOF-ready
Clone
8.1.1
Clonality
Monoclonal
Host
Golden Syrian Hamster
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Podoplanin Antibody (8.1.1) - BSA Free Summary

Background
Podoplanin is a mucine-type glycoprotein which induces platelet aggregation. Podoplanin has been implicated in cancer cell migration, invasion, metastasis, and malignant progression. Podoplanin antibodies serve as markers of lymphatic endothelium and fibroblastic reticular cells of lymphoid organs, as well as certain lung injuries. The upregulation of podoplanin in tumor cells has been cor...related with an increase in metastasis and motility. Recent research has shown that Podoplanin plays a critical role in immune cells, including T cells and dendritic cells.
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Immunogen
Murine thymic stromal cell lines
Localization
Membrane; Single-pass type I membrane protein.
Marker
Lymphatic Endothelium Marker
Isotype
IgG
Clonality
Monoclonal
Host
Golden Syrian Hamster
Gene
PDPN
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Electron Microscopy
  • Flow Cytometry 1:400
  • Immunocytochemistry/ Immunofluorescence 1-5ug/ml
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000-1:2000
Application Notes
Optimal dilutions/concentrations should be determined by the end user. This antibody is CyTOF ready.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB600-1015 in the following applications:

Publications
Read Publications using
NB600-1015 in the following applications:

Reactivity Notes

Does not cross-react with human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Podoplanin Antibody (8.1.1) - BSA Free

  • 36-KD
  • Aggrus
  • Gp36
  • Gp38
  • GP40
  • HT1A-1
  • hT1alpha-1
  • hT1alpha-2
  • lung type I cell membrane associated glycoprotein
  • lung type-I cell membrane-associated glycoprotein (T1A-2)
  • OTS 8
  • OTS8
  • PA2.26 antigen
  • PDPN
  • Podoplanin
  • RANDAM-2
  • T1A
  • T1A-2
  • T1-alpha

Background

Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a 38 kDa membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Podoplanin Antibody (NB600-1015)(21)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 8 applications: EM, FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
EM
(1)
FLOW
(1)
ICC/IF
(3)
IF/IHC
(1)
IHC
(1)
IHC-Fr
(2)
IHC-P
(8)
WB
(2)
All Applications
Filter By Species
Mouse
(15)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NB600-1015 Applications Species
Guo L, Bao W, Yang S et al. Rhei Radix et Rhizoma in Xuanbai-Chengqi decoction strengthens the intestinal barrier function and promotes lung barrier repair in preventing severe viral pneumonia induced by influenza A virus Journal of ethnopharmacology 2023-09-30 [PMID: 37783404] (IHC-P, Mouse)

Details:
1:100 dilution
IHC-P Mouse
Tang AT, Buchholz DW, Szigety KM et al. Cell-autonomous requirement for ACE2 across organs in lethal mouse SARS-CoV-2 infection PLoS biology 2023-02-01 [PMID: 36745682] (IHC, Mouse) IHC Mouse
Fraser K, Hubbs A, Yanamala N et al. Histopathology of the broad class of carbon nanotubes and nanofibers used or produced in U.S. facilities in a murine model Particle and fibre toxicology 2021-12-20 [PMID: 34923995] (IHC-P, Mouse) IHC-P Mouse
Shan Z, Li L, Atkins CL et al. Chitinase 3-like-1 contributes to acetaminophen-induced liver injury by promoting hepatic platelet recruitment eLife 2021-06-10 [PMID: 34110284] (IHC-P, Mouse) IHC-P Mouse
Neto LMM, Zufelato N, de Sousa-Junior AA et al. Specific T cell induction using iron oxide based nanoparticles as subunit vaccine adjuvant Hum Vaccin Immunother 2018-06-18 [PMID: 29913109] (Mouse) Mouse
Asfahani RI, Tahoun MM, Miller-Hodges EV et al. Activation of podocyte Notch mediates early Wt1 glomerulopathy. kidney Int. 2018-04-01 [PMID: 29398135] (ICC/IF, Mouse) ICC/IF Mouse
Jia Y, Chen K, Lin P et al. Treatment of acute lung injury by targeting MG53-mediated cell membrane repair. Nat Commun 2014-07-18 [PMID: 25034454] (IHC-P, Mouse)

Details:
Podoplanin/AT1 alpha antibody used in IHC-P as a specific cell marker for type I alveolar epithelial cells in lungs of wild-type and mg53-/- mice - tissue fixed in 4% neutral-buffered paraformaldehyde for 24 hous at 4C, paraffin blocks cut into 4um sections, staining detection with IF labelled secondary (Figure 1e and 1f; Supplementary Figure 2).
IHC-P Mouse
Sargent LM, Porter DW, Staska LM et al. Promotion of lung adenocarcinoma following inhalation exposure to multi-walled carbon nanotubes. Part Fibre Toxicol 2014-01-15 [PMID: 24405760] (IHC-P, Mouse) IHC-P Mouse
Kabgani N, Grigoleit T, Schulte K, Sechi A, Sauer-Lehnen S, Tag C, Boor P, Kuppe C, Warsow G, Schordan S, Mostertz J, Chilukoti RK, Homuth G, Endlich N, Tacke F, Weiskirchen R, Fuellen G, Endlich K, Floege J, Smeets B, Moeller MJ. Primary cultures of glomerular parietal epithelial cells or podocytes with proven origin. PLoS One;7(4). 2012-01-01 [PMID: 22529955] (WB, Mouse) WB Mouse
Porter DW, Hubbs AF, Mercer RR et al. Mouse pulmonary dose- and time course-responses induced by exposure to multi-walled carbon nanotubes. Toxicology 2010-03-10 [PMID: 19857541] (ICC/IF, IF/IHC, Mouse) ICC/IF, IF/IHC Mouse
Show All 21 Publications.

Review for Podoplanin Antibody (NB600-1015) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB600-1015:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Podoplanin NB600-1015
Enlarge
5
reviewed by:
Verified Customer
IHC-P Mouse 05/17/2011
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse mammary gland after MDA-MB-231 orthotopic transplantation
SpeciesMouse
LotA1

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Podoplanin Antibody (NB600-1015) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on Podoplanin.

Spheroids vs. Organoids: Which 3D Cell Culture Model is Best for You?
By Jennifer Jones, M.S.Spheroids and organoids are two words that, like “butter” and “margarine”, are often referred to interchangeably but have distinct meanings. The progression and adopt...  Read full blog post.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein)
Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with ...  Read full blog post.

mFluor Violet Conjugated Antibodies

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Recent Reviews

5
5
1
4
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3
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2
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1
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Verified Customer
05/17/2011
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol PDPN
Entrez
Uniprot