Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) [NB600-1015] - Staining for podoplanin (red) and cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous mesothelioma. In ...read more
Western Blot: Podoplanin Antibody (8.1.1) [NB600-1015] - Podoplanin is differentially expressed in primary podocytes relative to primary PECs after six passages in culture (arrow), consistent with mRNA expression ...read more
Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) [NB600-1015] - Analysis of Podoplanin in mouse mammary gland after MDA-MB-231 orthotopic transplantation. Image courtesy of product review by Luana Schito.
Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) [NB600-1015] - Staining for podoplanin (red) and cytokeratins (green) in malignant serosal tumors morphologically consistent with sarcomatous mesothelioma. In ...read more
Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) [NB600-1015] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The ...read more
Western Blot: Podoplanin Antibody (8.1.1) [NB600-1015] - Analysis of Podoplanin in mouse kidney tissue extract.
Immunohistochemistry: Podoplanin Antibody (8.1.1) [NB600-1015] - Podoplanin (8.1.1) antibody labeling (Green) of glomeruli from mouse kidney. Nuclei were counterstained with DAPI (Blue).
Podoplanin is a mucine-type glycoprotein which induces platelet aggregation. Podoplanin has been implicated in cancer cell migration, invasion, metastasis, and malignant progression. Podoplanin antibodies serve as markers of lymphatic endothelium and fibroblastic reticular cells of lymphoid organs, as well as certain lung injuries. The upregulation of podoplanin in tumor cells has been cor...related with an increase in metastasis and motility. Recent research has shown that Podoplanin plays a critical role in immune cells, including T cells and dendritic cells.
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Immunogen
Murine thymic stromal cell lines
Localization
Membrane; Single-pass type I membrane protein.
Marker
Lymphatic Endothelium Marker
Isotype
IgG
Clonality
Monoclonal
Host
Golden Syrian Hamster
Gene
PDPN
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Optimal dilutions/concentrations should be determined by the end user. This antibody is CyTOF ready.
Theoretical MW
40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5 using NB600-1015 in the following applications:
Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a 38 kDa membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Bioinformatics Tool for Podoplanin Antibody (NB600-1015)
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Diseases for Podoplanin Antibody (NB600-1015)
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