Podoplanin Antibody (8.1.1)

Western Blot: Podoplanin Antibody (8.1.1) [NB600-1015] - Analysis of Podoplanin in mouse kidney tissue extract.

Immunocytochemistry/Immunofluorescence: Podoplanin Antibody (8.1.1) [NB600-1015] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- at 5 ug/ml overnight at 4C and detected with an anti-Golden Syrian hamster DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) [NB600-1015] - Analysis of Podoplanin in mouse mammary gland after MDA-MB-231 orthotopic transplantation. Image courtesy of product review by Luana Schito.

Immunocytochemistry/Immunofluorescence: Podoplanin Antibody (8.1.1) [NB600-1015] - Podoplanin (8.1.1) antibody labeling (Green) of glomeruli from mouse kidney. Nuclei were counterstained with DAPI (Blue).

• Reactivity: Mu, Hu(-)
• Applications: WB, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
• Clone: 8.1.1
• Clonality: Monoclonal
• Host: Golden Syrian Hamster
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Podoplanin Antibody (8.1.1) Summary

• Background: Podoplanin is a mucine-type glycoprotein which induces platelet aggregation. Podoplanin has been implicated in cancer cell migration, invasion, metastasis, and malignant progression. Podoplanin antibodies serve as markers of lymphatic endothelium and fibroblastic reticular cells of lymphoid organs, as well as certain lung injuries. The upregulation of podoplanin in tumor cells has been cor...related with an increase in metastasis and motility. Recent research has shown that Podoplanin plays a critical role in immune cells, including T cells and dendritic cells.
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• Immunogen: Murine thymic stromal cell lines
• Localization: Membrane; Single-pass type I membrane protein.
• Marker: Lymphatic Endothelium Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Golden Syrian Hamster
• Gene: PDPN
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:2000
  • Electron Microscopy
  • Flow Cytometry 1:400
  • Immunocytochemistry/Immunofluorescence 1-5ug/ml
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
• Application Notes: In Western blot, a band can be seen at ~40 kDa. Optimal dilutions/concentrations should be determined by the end user. This antibody is CyTOF ready.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Does not cross-react with human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Podoplanin Antibody (8.1.1)

• 36-KD
• Aggrus
• Gp36
• Gp38
• GP40
• HT1A-1
• hT1alpha-1
• hT1alpha-2
• lung type I cell membrane associated glycoprotein
• lung type-I cell membrane-associated glycoprotein (T1A-2)
• OTS 8
• OTS8
• PA2.26 antigen
• PDPN
• Podoplanin
• RANDAM-2
• T1A
• T1A-2
• T1-alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Podoplanin Antibody (NB600-1015)(17)

Publications using NB600-1015

• Neto LMM, Zufelato N, de Sousa-Junior AA et al. Specific T cell induction using iron oxide based nanoparticles as subunit vaccine adjuvant Hum Vaccin Immunother Jun 18 2018 [PMID: 29913109] (Mouse)
• Asfahani RI, Tahoun MM, Miller-Hodges EV et al. Activation of podocyte Notch mediates early Wt1 glomerulopathy. kidney Int. Apr 1 2018 [PMID: 29398135] (ICC/IF, Mouse)
• Jia Y, Chen K, Lin P et al. Treatment of acute lung injury by targeting MG53-mediated cell membrane repair. Nat Commun 2014 Jul 18 [PMID: 25034454] (IHC-P, Mouse)
  
  Details:
  Podoplanin/AT1 alpha antibody used in IHC-P as a specific cell marker for type I alveolar epithelial cells in lungs of wild-type and mg53-/- mice - tissue fixed in 4% neutral-buffered paraformaldehyde for 24 hous at 4C, paraffin blocks cut into 4um sections, staining detection with IF labelled secondary (Figure 1e and 1f; Supplementary Figure 2).
• Sargent LM, Porter DW, Staska LM et al. Promotion of lung adenocarcinoma following inhalation exposure to multi-walled carbon nanotubes. Part Fibre Toxicol 2014 Jan 15 [PMID: 24405760] (IHC-P, Mouse)
• Kabgani N, Grigoleit T, Schulte K, Sechi A, Sauer-Lehnen S, Tag C, Boor P, Kuppe C, Warsow G, Schordan S, Mostertz J, Chilukoti RK, Homuth G, Endlich N, Tacke F, Weiskirchen R, Fuellen G, Endlich K, Floege J, Smeets B, Moeller MJ. Primary cultures of glomerular parietal epithelial cells or podocytes with proven origin. PLoS One;7(4). 2012 [PMID: 22529955] (WB, Mouse)
• Porter DW, Hubbs AF, Mercer RR et al. Mouse pulmonary dose- and time course-responses induced by exposure to multi-walled carbon nanotubes. Toxicology 2010 Mar 10 [PMID: 19857541] (IHC, ICC/IF, Mouse)
• Mahtab EA, Vicente-Steijn R, Hahurij ND, Jongbloed MR, Wisse LJ, DeRuiter MC, Uhrin P, Zaujec J, Binder BR, Schalij MJ, Poelmann RE, Gittenberger-de Groot AC. Podoplanin deficient mice show a RhoA-related hypoplasia of the sinus venosus myocardium including the sinoatrial node. Dev Dyn238(1):183-93. 2009 Jan. [PMID: 19097191]
• Mahtab EA, Wijffels MC, Van Den Akker NM, Hahurij ND, Lie-Venema H, Wisse LJ, Deruiter MC, Uhrin P, Zaujec J, Binder BR, Schalij MJ, Poelmann RE, Gittenberger-De Groot AC. Cardiac malformations and myocardial abnormalities in podoplanin knockout mouse embryos: Correlation with abnormal epicardial development. Dev Dyn237(3):847-57. 2008 Mar. [PMID: 18265012]
• Withers DR, Kim MY, Bekiaris V, Rossi SW, Jenkinson WE, Gaspal F, McConnell F, Caamano JH, Anderson G, Lane PJ. The role of lymphoid tissue inducer cells in splenic white pulp development. Eur J Immunol37(11):3240-5. 2007 Nov. [PMID: 17948268]
• Gittenberger-de Groot AC, Mahtab EA, Hahurij ND, Wisse LJ, Deruiter MC, Wijffels MC, Poelmann RE. Nkx2.5-negative myocardium of the posterior heart field and its correlation with podoplanin expression in cells from the developing cardiac pacemaking and conduction system. Anat Rec. 2007 Jan [PMID: 17441204]
• Hara T, Katakai T, Lee JH, Nambu Y, Nakajima-Nagata N, Gonda H, Sugai M, Shimizu A. A transmembrane chemokine, CXC chemokine ligand 16, expressed by lymph node fibroblastic reticular cells has the potential to regulate T cell migration and adhesion. Int Immunol;18(2):301-11. 2006 Feb. [PMID: 16410312]
• Schacht V, Dadras SS, Johnson LA, Jackson DG, Hong YK, Detmar M. Up regulation of the lymphatic marker podoplanin, a mucin-type transmembrane glycoprotein, in human squamous cell carcinomas and germ cell tumors. Am J Pathol;166(3):913-21. 2005 Mar. [PMID: 15743802] (IHC-P, Mouse)
• Crnic I, Strittmatter K, Cavallaro U, Kopfstein L, Jussila L, Alitalo K, Christofori G. Loss of neural cell adhesion molecule induces tumor metastasis by up-regulating lymphangiogenesis. Cancer Res;64(23):8630-8. 2004 Dec 1. [PMID: 15574770] (IHC-P, Mouse)
• Katakai T, Hara T, Sugai M et al. Lymph node fibroblastic reticular cells construct the stromal reticulum via contact with lymphocytes. J Exp Med;200(6):783-95. 2004 Sep 20. [PMID: 15381731] (FLOW, IHC-Fr, ICC/IF, Mouse)
• Schacht V, Ramirez MI, Hong YK et al. T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema. EMBO J;22(14):3546-56. 2003 Jul 15. [PMID: 12853470] (IHC-P, EM, Mouse)
• Farr AG, Berry ML, Kim A, Nelson AJ, Welch MP, Aruffo A. Characterization and cloning of a novel glycoprotein expressed by stromal cells in T-dependent areas of peripheral lymphoid tissues. J Exp Med;176(5):1477-82. 1992 Nov 1. [PMID: 1402691] (WB, IHC-Fr, Mouse)
• Farr A, Nelson A, Hosier S. Characterization of an antigenic determinant preferentially expressed by type I epithelial cells in the murine thymus. J Histochem Cytochem;40(5):651-64. 1992 May. [PMID: 1374092]

Review for Podoplanin Antibody (NB600-1015) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous IHC-P Mouse 05/17/2011

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse mammary gland after MDA-MB-231 orthotopic transplantation
• Species: Mouse
• Lot: A1
• Comments: Highly specific staining of the mouse lymphatic vessels only. No background, no staining of blood vessels.

Blocking

• Blocking Details: Blocking Buffer: Protein block solution (Dako), Blocking Time: 10 minutes, Blocking Temp: 21 degrees Celsius

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:100, Incubation Dilution Buffer: PBS-0.1% Tween-20,Incubation Time: 30min, Incubation Temp: 21C

Secondary Antibody

• Secondary Description: Secondary Ab: Labeled streptavidin-biotin-HRP system
• Secondary Manufacturer Cat#: Dako, cat#: K0679

Details

• Detection Notes: Detection Method:DAB, Pos Ctrl:Mouse spleen,Deparaffinization:xylene & ethanol, Antigen Retrieval:Sodium citrate pH6, 30min@100C
• Fixation Details: Sample Preparation: formalin fixed & paraffin embedded, Deparaffinization: xylene and ethanol

Comments

• Comments: Highly specific staining of the mouse lymphatic vessels only. No background, no staining of blood vessels.

Bioinformatics

• Gene Symbol: PDPN
• Entrez:
  • 14726(Mouse)
• Uniprot:
  • Q62011(Mouse)