Western Blot: Podoplanin Antibody (8.1.1) [NB600-1015] - Analysis of Podoplanin in mouse kidney tissue extract.
Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) [NB600-1015] - Podoplanin (8.1.1) antibody labeling (green) of glomeruli from mouse kidney. Nuclei were counterstained with Dapi (blue).
Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) [NB600-1015] - Analysis of Podoplanin in mouse mammary gland after MDA-MB-231 orthotopic transplantation. Image courtesy of product review by Luana Schito.
Podoplanin is a mucine-type glycoprotein which induces platelet aggregation. Podoplanin has been implicated in cancer cell migration, invasion, metastasis, and malignant progression. Podoplanin antibodies serve as markers of lymphatic endothelium and fibroblastic reticular cells of lymphoid organs, as well as certain lung injuries. The upregulation of podoplanin in tumor cells has been cor...related with an increase in metastasis and motility. Recent research has shown that Podoplanin plays a critical role in immune cells, including T cells and dendritic cells.
Murine thymic stromal cell lines
Membrane; Single-pass type I membrane protein.
Lymphatic Endothelium Marker
Golden Syrian Hamster
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Protein G purified
Western Blot 1:1000-1:2000
Flow Cytometry 1:400
This Podoplanin (8.1.1) antibody is useful in Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections. Electron Microscopy and Immunohistochemistry-Frozen were reported in scientific literature. Immunoprecipitation and Western Blot, where a band can be seen at ~40 kDa. Optimal dilutions/concentrations should be determined by the end user.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NB600-1015 in the following applications: