Podoplanin Antibody (8.1.1)

Images

 
Western Blot: Podoplanin Antibody (8.1.1) [NB600-1015] - Analysis of Podoplanin in mouse kidney tissue extract.
Immunocytochemistry/ Immunofluorescence: Podoplanin Antibody (8.1.1) [NB600-1015] - Podoplanin (8.1.1) antibody labeling (green) of glomeruli from mouse kidney. Nuclei were counterstained with Dapi (blue).
Immunohistochemistry-Paraffin: Podoplanin Antibody (8.1.1) [NB600-1015] - Analysis of Podoplanin in mouse mammary gland after MDA-MB-231 orthotopic transplantation. Image courtesy of product review by Luana Schito.

Product Details

Summary
Reactivity Mu, Hu(-)Species Glossary
Applications WB, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
8.1.1
Clonality
Monoclonal
Host
Golden Syrian Hamster
Conjugate
Unconjugated
Concentration
1 mg/ml

Podoplanin Antibody (8.1.1) Summary

Background
Podoplanin is a mucine-type glycoprotein which induces platelet aggregation. Podoplanin has been implicated in cancer cell migration, invasion, metastasis, and malignant progression. Podoplanin antibodies serve as markers of lymphatic endothelium and fibroblastic reticular cells of lymphoid organs, as well as certain lung injuries. The upregulation of podoplanin in tumor cells has been cor...related with an increase in metastasis and motility. Recent research has shown that Podoplanin plays a critical role in immune cells, including T cells and dendritic cells.
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Immunogen
Murine thymic stromal cell lines
Localization
Membrane; Single-pass type I membrane protein.
Marker
Lymphatic Endothelium Marker
Isotype
IgG
Clonality
Monoclonal
Host
Golden Syrian Hamster
Gene
PDPN
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Electron Microscopy
  • Flow Cytometry 1:400
  • Immunocytochemistry/Immunofluorescence 1-5ug/ml
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Application Notes
This Podoplanin (8.1.1) antibody is useful in Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections. Electron Microscopy and Immunohistochemistry-Frozen were reported in scientific literature. Immunoprecipitation and Western Blot, where a band can be seen at ~40 kDa. Optimal dilutions/concentrations should be determined by the end user. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB600-1015 in the following applications:

Publications
Read Publications using
NB600-1015 in the following applications:

Reactivity Notes

Mouse. Does not cross-react with human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Podoplanin Antibody (8.1.1)

  • 36-KD
  • Aggrus
  • Gp36
  • Gp38
  • GP40
  • HT1A-1
  • hT1alpha-1
  • hT1alpha-2
  • lung type I cell membrane associated glycoprotein
  • lung type-I cell membrane-associated glycoprotein (T1A-2)
  • OTS 8
  • OTS8
  • PA2.26 antigen
  • PDPN
  • Podoplanin
  • RANDAM-2
  • T1A
  • T1A-2
  • T1-alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Podoplanin Antibody (NB600-1015)(16)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 7 applications: EM, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
EM
(1)
FLOW
(1)
ICC/IF
(2)
IHC
(1)
IHC-Fr
(2)
IHC-P
(5)
WB
(2)
All Applications
Filter By Species
Mouse
(10)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB600-1015 Applications Species
Neto LMM, Zufelato N, de Sousa-Junior AA et al. Specific T cell induction using iron oxide based nanoparticles as subunit vaccine adjuvant Hum Vaccin Immunother Jun 18 2018 [PMID: 29913109] (Mouse) Mouse
Jia Y, Chen K, Lin P et al. Treatment of acute lung injury by targeting MG53-mediated cell membrane repair. Nat Commun 2014 Jul 18 [PMID: 25034454] (IHC-P, Mouse)

Details:
Podoplanin/AT1 alpha antibody used in IHC-P as a specific cell marker for type I alveolar epithelial cells in lungs of wild-type and mg53-/- mice - tissue fixed in 4% neutral-buffered paraformaldehyde for 24 hous at 4C, paraffin blocks cut into 4um sections, staining detection with IF labelled secondary (Figure 1e and 1f; Supplementary Figure 2).
IHC-P Mouse
Sargent LM, Porter DW, Staska LM et al. Promotion of lung adenocarcinoma following inhalation exposure to multi-walled carbon nanotubes. Part Fibre Toxicol 2014 Jan 15 [PMID: 24405760] (IHC-P, Mouse) IHC-P Mouse
Kabgani N, Grigoleit T, Schulte K, Sechi A, Sauer-Lehnen S, Tag C, Boor P, Kuppe C, Warsow G, Schordan S, Mostertz J, Chilukoti RK, Homuth G, Endlich N, Tacke F, Weiskirchen R, Fuellen G, Endlich K, Floege J, Smeets B, Moeller MJ. Primary cultures of glomerular parietal epithelial cells or podocytes with proven origin. PLoS One;7(4). 2012 [PMID: 22529955] (WB, Mouse) WB Mouse
Porter DW, Hubbs AF, Mercer RR et al. Mouse pulmonary dose- and time course-responses induced by exposure to multi-walled carbon nanotubes. Toxicology 2010 Mar 10 [PMID: 19857541] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Mahtab EA, Vicente-Steijn R, Hahurij ND, Jongbloed MR, Wisse LJ, DeRuiter MC, Uhrin P, Zaujec J, Binder BR, Schalij MJ, Poelmann RE, Gittenberger-de Groot AC. Podoplanin deficient mice show a RhoA-related hypoplasia of the sinus venosus myocardium including the sinoatrial node. Dev Dyn238(1):183-93. 2009 Jan. [PMID: 19097191]
Mahtab EA, Wijffels MC, Van Den Akker NM, Hahurij ND, Lie-Venema H, Wisse LJ, Deruiter MC, Uhrin P, Zaujec J, Binder BR, Schalij MJ, Poelmann RE, Gittenberger-De Groot AC. Cardiac malformations and myocardial abnormalities in podoplanin knockout mouse embryos: Correlation with abnormal epicardial development. Dev Dyn237(3):847-57. 2008 Mar. [PMID: 18265012]
Withers DR, Kim MY, Bekiaris V, Rossi SW, Jenkinson WE, Gaspal F, McConnell F, Caamano JH, Anderson G, Lane PJ. The role of lymphoid tissue inducer cells in splenic white pulp development. Eur J Immunol37(11):3240-5. 2007 Nov. [PMID: 17948268]
Gittenberger-de Groot AC, Mahtab EA, Hahurij ND, Wisse LJ, Deruiter MC, Wijffels MC, Poelmann RE. Nkx2.5-negative myocardium of the posterior heart field and its correlation with podoplanin expression in cells from the developing cardiac pacemaking and conduction system. Anat Rec. 2007 Jan [PMID: 17441204]
Hara T, Katakai T, Lee JH, Nambu Y, Nakajima-Nagata N, Gonda H, Sugai M, Shimizu A. A transmembrane chemokine, CXC chemokine ligand 16, expressed by lymph node fibroblastic reticular cells has the potential to regulate T cell migration and adhesion. Int Immunol;18(2):301-11. 2006 Feb. [PMID: 16410312]
Show All 16 Publications.

Review for Podoplanin Antibody (NB600-1015) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB600-1015:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Podoplanin NB600-1015
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reviewed by:
Anonymous
IHC-P Mouse 05/17/2011
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse mammary gland after MDA-MB-231 orthotopic transplantation
SpeciesMouse
LotA1
CommentsHighly specific staining of the mouse lymphatic vessels only. No background, no staining of blood vessels.

Blocking

Blocking DetailsBlocking Buffer: Protein block solution (Dako), Blocking Time: 10 minutes, Blocking Temp: 21 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:100, Incubation Dilution Buffer: PBS-0.1% Tween-20,Incubation Time: 30min, Incubation Temp: 21C

Secondary Antibody

Secondary DescriptionSecondary Ab: Labeled streptavidin-biotin-HRP system
Secondary Manufacturer Cat#Dako, cat#: K0679

Details

Detection NotesDetection Method:DAB, Pos Ctrl:Mouse spleen,Deparaffinization:xylene & ethanol, Antigen Retrieval:Sodium citrate pH6, 30min@100C
Fixation DetailsSample Preparation: formalin fixed & paraffin embedded, Deparaffinization: xylene and ethanol

Comments

CommentsHighly specific staining of the mouse lymphatic vessels only. No background, no staining of blood vessels.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Podoplanin Antibody (NB600-1015) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-1015AF405
Alexa Fluor 488 Labeled NB600-1015AF488
Alexa Fluor 647 Labeled NB600-1015AF647
Alexa Fluor 700 Labeled NB600-1015AF700
Allophycocyanin Labeled NB600-1015APC
Biotin Labeled NB600-1015B
DyLight 350 Labeled NB600-1015UV
DyLight 405 Labeled NB600-1015V
DyLight 488 Labeled NB600-1015G
DyLight 550 Labeled NB600-1015R
DyLight 650 Labeled NB600-1015C
DyLight 680 Labeled NB600-1015FR
DyLight 755 Labeled NB600-1015IR
FITC Labeled NB600-1015F
HRP Labeled NB600-1015H
PE Labeled NB600-1015PE
PerCP Labeled NB600-1015PCP

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Bioinformatics Tool for Podoplanin Antibody (NB600-1015)

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Blogs on Podoplanin.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein)
Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with...  Read full blog post.

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Recent Reviews

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1
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Anonymous
05/17/2011
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol PDPN
Entrez
Uniprot