Podocalyxin Like Antibody

Western Blot: Podocalyxin Antibody [NB100-60950] - (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Discontinued

Podocalyxin Like Antibody Summary

• Immunogen: Peptide with sequence C-DNLTKDDLDEEEDTH corresponding to C-Terminus according to NP_005388.2.
• Epitope: DNLTKDDLDEEEDTH
• Marker: Podocytes /Apical Marker, Endothelial Cell Marker
• Specificity: This antibody is expected to recognise both reported isoforms (NP_001018121.1 and NP_005388.2).
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PODXL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 160 kDa band observed in human, mouse and rat kidney lysates is reported in scientific literature (PMID: 9188463).
• Control:

  Podocalyxin Like Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Podocalyxin Like Antibody

• GCTM
• Gp200MGC138240
• PCLP
• PCLP1
• PCLP-1
• PCLPGCTM-2 antigen
• PCpodocalyxin
• POD XL
• Podocalyxin
• Podocalyxin-like protein 1
• podocalyxin-like
• PODXL

Background

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Podocalyxin Like Antibody (NB100-60950)(1)

Publication using NB100-60950

• Nielsen JS, Graves ML, Chelliah S et al. The CD34-Related Molecule Podocalyxin Is a Potent Inducer of Microvillus Formation. PLoS ONE 2007-02-21 [PMID: 17311105]

Bioinformatics

• Gene Symbol: PODXL
• Entrez:
  • 5420(Human)
  • 27205(Mouse)
  • 192181(Rat)
• Uniprot:
  • NP_001018121.1(Human)