Podocalyxin Like Antibody (PODXL/2185)

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (PODXL/2185) [NBP2-79749] - Formalin-fixed, paraffin-embedded human Endometrium stained with Podocalyxin Like Antibody (PODXL/2185).

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (PODXL/2185) [NBP2-79749] - Formalin-fixed, paraffin-embedded human Placenta stained with Podocalyxin Like Antibody (PODXL/2185).

Protein Array: Podocalyxin Like Antibody (PODXL/2185) [NBP2-79749] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Podocalyxin Like Antibody (PODXL/2185). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

• Reactivity: Hu
• Applications: IHC, MA
• Clone: PODXL/2185
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Podocalyxin Like Antibody (PODXL/2185) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79884)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant fragment of human Podocalyxin Like (around aa 310-447) (exact sequence is proprietary) (Uniprot: O00592)
• Localization: Cell Surface, Cytoplasm
• Marker: Hematopoietic Stem Cell Marker
• Specificity: Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PODXL
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Podocalyxin Like Antibody (PODXL/2185)

• GCTM
• Gp200MGC138240
• PCLP
• PCLP1
• PCLP-1
• PCLPGCTM-2 antigen
• PCpodocalyxin
• POD XL
• Podocalyxin
• Podocalyxin-like protein 1
• podocalyxin-like
• PODXL

Background

Podocalyxin (PODXL) is a CD34 family member expressed by podocytes, vascular endothelium, mesothelium, and a subset of hematopoietic progenitors. It is the major sialoprotein in the glycocalyx of glomerular podocytes. The physiological role of podocalyxin in leukemic blasts is unknown. However, it is thought of as a candidate prostate cancer tumor aggressiveness gene. Podocalyxin appears to complement CD34 as a useful cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Podocalyxin Like Antibody (NBP2-79749)(1)

Publication using NBP2-79749

• Lin Z, Zhang X, Fritch MR Et al. Engineering pre-vascularized bone-like tissue from human mesenchymal stem cells through simulating endochondral ossification Biomaterials 2022-03-08 [PMID: 35259584] (IHC-P, Human)
  
  Details:
  Citation using the Alexa Fluor 594 version of this antibody.

Bioinformatics

• Gene Symbol: PODXL
• Uniprot:
  • O00592(Human)