Podocalyxin Like Antibody (5F5)

Western Blot: Podocalyxin Like Antibody (5F5) [NBP2-37605] - Western blot analysis using PODXL mAb against human PODXL (AA: 109-324) recombinant protein. (Expected MW is 47.3 kDa)

Immunohistochemistry: Podocalyxin Like Antibody (5F5) [NBP2-37605] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney tissues (right) using PODXL mouse mAb with DAB staining.

Flow Cytometry: Podocalyxin Like Antibody (5F5) [NBP2-37605] - Flow cytometric analysis of Hela cells using PODXL mouse mAb (green) and negative control (purple).

ELISA: Podocalyxin Like Antibody (5F5) [NBP2-37605] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

Podocalyxin Like Antibody (5F5) Summary

• Immunogen: Purified recombinant fragment of human PODXL expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PODXL
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Podocalyxin Like Antibody (5F5)

• GCTM
• Gp200MGC138240
• PCLP
• PCLP1
• PCLP-1
• PCLPGCTM-2 antigen
• PCpodocalyxin
• POD XL
• Podocalyxin
• Podocalyxin-like protein 1
• podocalyxin-like
• PODXL

Background

Podocalyxin (PODXL) is a CD34 family member expressed by podocytes, vascular endothelium, mesothelium, and a subset of hematopoietic progenitors. It is the major sialoprotein in the glycocalyx of glomerular podocytes. The physiological role of podocalyxin in leukemic blasts is unknown. However, it is thought of as a candidate prostate cancer tumor aggressiveness gene. Podocalyxin appears to complement CD34 as a useful cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PODXL