Western Blot: Podocalyxin Antibody (3D3) [NBP2-25219] - HeLa lysate probed with Podocalyxin antibody at 3 ug/ml.
Immunohistochemistry-Paraffin: Podocalyxin Antibody (3D3) [NBP2-25219] - Formalin-paraffin angiosarcoma stained with Podocalyxin Ab (3D3).
Flow Cytometry: Podocalyxin Antibody (3D3) [NBP2-25219] - Surface staining of NCCIT cells using Podocalyxin antibody at 1 ug/10^6 cells. A FITC-conjugated anti mouse Ig secondary antibody was used along with this ...read more
Flow Cytometry: Podocalyxin Antibody (3D3) [NBP2-25219] - Analysis using PE conjugate of NBP2-25219. A cell surface stain was performed on HeLa cells with Podocalyxin antibody (3D3) NBP2-33108 (blue) and a matched ...read more
Hu, Rt, RbSpecies Glossary
WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
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A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin was used as the immunogen to generate the 3D3, 4F10 and 2A4 podocalyxin antibody clones (Karshaw, 1997). The 3D3 antibody recognizes an epitope in the extracellular domain, and both the 4F10 and 2A4 antibody clones recognize epitopes in the intracellular domain of podocalyxin.
Cell surface and Cytoplasmic
Podocytes /Apical Marker, Endothelial Cell Marker
Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.
Protein A or G purified
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Alternate Names for Podocalyxin Like Antibody (3D3)
Podocalyxin-like protein 1
Podocalyxin, also known as podocalyxin-like or PDXL, is a member of the CD34 transmembrane sialomucin family. Like other sialoglycoproteins, podocalyxin plays important roles in mediating cellular migration and cell-cell adhesion. Podocalyxin was first discovered in podocytes, the terminally differentiated epithelial cells lining the kidney glomeruli. Podocalyxin is expressed at high levels on the podocyte foot projections and plays essential roles in kidney development and homeostasis, particularly blood filtration and urine formation. Podocalyxin is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons (reviewed in Nielsen, 2009). Aberrant expression of podocalyxin has been described in a variety of cancers including breast, prostate, liver, pancreatic, kidney and leukemia. Human podocalyxin migrates as a 165/170 kDa doublet on SDS-PAGE; however there are many post-translational modifications which may impact its actual size (Kershaw, 1997). Antibody to podocalyxin has been reported to be a useful biomarker for various pathological conditions. For example, podocalyxin antibody can identify podocytes in the urine, also known as podocyturia, which can be indicative of glomerular disease, pre-eclampsia, and other kidney pathology (reviewed in Chen, 2012). Additionally, podocalyxin antibody studies have indicated that highly podocalyxin-positive tumors or podocalyxin dysregulation in general can be potentially linked to more aggressive tumors and poorer outcomes (Nielson, 2009). Hence, podocalyxin antibody may be used as part of a biomarker antibody panel to study expression of proteins associated with tumorigenesis. The podocalyxin antibody clones 3D3, 4F10, and 2A4 have been extensively published in the literature and researchers are encouraged to consult the published literature for additional information.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.