Podocalyxin Like Antibody (222328) [Allophycocyanin]

BG01V human embryonic stem cells were stained with Mouse Anti-Human Podocalyxin APC-conjugated Monoclonal Antibody (Catalog # FAB1658A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

Podocalyxin Like Antibody (222328) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Podocalyxin
  Ser23-Arg427
  Accession # AAB61574
• Specificity: Detects human Podocalyxin in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Podocalyxin or recombinant human Endoglycan is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PODXL
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Podocalyxin Like Antibody (222328) [Allophycocyanin]

• GCTM
• Gp200MGC138240
• PCLP
• PCLP1
• PCLP-1
• PCLPGCTM-2 antigen
• PCpodocalyxin
• POD XL
• Podocalyxin
• Podocalyxin-like protein 1
• podocalyxin-like
• PODXL

Background

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology (1). Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors (2). It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

1. Li, J. et al. (2001) DNA Seq. 12:407.
2. Hara, T. et al. (1999) Immunity 11:567.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Podocalyxin Like Antibody (FAB1658A)(1)

Publication using FAB1658A

• AD Westerling, EM Fast, TW Soare, S Venkatacha, M DeRan, AB Fanelli, S Kyrychenko, H Hoang, GM Corriea, W Zhang, M Yu, M Daniels, G Malojcic, XR Pan-Zhou, MW Ledeboer, JC Harmange, M Emani, TT Tibbitts, JF Reilly, P Mundel Transplanted organoids empower human preclinical assessment of drug candidate for the clinic Science Advances, 2022-07-06;8(27):eabj5633. 2022-07-06 [PMID: 35857479]

Bioinformatics

• Gene Symbol: PODXL
• Uniprot:
  • AAB61574()