PMEL17/SILV Antibody (HMB45) [Alexa Fluor® 594]


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Product Details

Reactivity Hu, Ca(-), Rt(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Alexa Fluor 594

Order Details

PMEL17/SILV Antibody (HMB45) [Alexa Fluor® 594] Summary

Extract of pigmented melanoma metastases from lymph nodes
By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).
IgG1 Kappa
Protein A or G purified
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  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
Protein A or G purified


Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PMEL17/SILV Antibody (HMB45) [Alexa Fluor® 594]

  • D12S53EP1
  • gp100
  • ME20
  • ME20-M
  • melanocyte protein mel 17
  • Melanocyte protein Pmel 17
  • Melanocytes lineage-specific antigen GP100
  • Melanoma-associated ME20 antigen
  • melanosomal matrix protein17
  • PMEL17P100
  • premelanosome proteinME20M
  • SI
  • SIL
  • silver (mouse homolog) like
  • silver homolog (mouse)
  • Silver locus protein homolog
  • silver, mouse, homolog of
  • SILVPmel17


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PMEL17/SILV Antibody (NBP2-34638AF594) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34638AF350
Alexa Fluor 405 NBP2-34638AF405
Alexa Fluor 488 NBP2-34638AF488
Alexa Fluor 594 NBP2-34638AF594
Alexa Fluor 647 NBP2-34638AF647
Alexa Fluor 700 NBP2-34638AF700
Alexa Fluor 750 NBP2-34638AF750
Allophycocyanin NBP2-34638APC
Allophycocyanin/Cy7 NBP2-34638APCCY7
Biotin NBP2-34638B
DyLight 350 NBP2-34638UV
DyLight 405 NBP2-34638V
DyLight 488 NBP2-34638G
DyLight 550 NBP2-34638R
DyLight 594 NBP2-34638DL594
DyLight 650 NBP2-34638C
DyLight 680 NBP2-34638FR
DyLight 755 NBP2-34638IR
HRP NBP2-34638H
Janelia Fluor 549 NBP2-34638JF549
Janelia Fluor 646 NBP2-34638JF646
PE NBP2-34638PE
PE/Atto594 NBP2-34638PEATT594
PE/Cy5.5 NBP2-34638PECY55
PE/Cy7 NBP2-34638PECY7
PerCP NBP2-34638PCP

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Gene Symbol PMEL