PMEL17/SILV Antibody (HMB45) [Alexa Fluor® 405]

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Product Details

Summary
Reactivity Hu, Ca(-), Rt(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Clone
HMB45
Clonality
Monoclonal
Host
Mouse

PMEL17/SILV Antibody (HMB45) [Alexa Fluor® 405] Summary

Immunogen
Extract of pigmented melanoma metastases from lymph nodes
Localization
Cytoplasmic
Marker
Melanoma Marker
Specificity
By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SILV, PMEL
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
Alexa Fluor 405
Format
Alexa Fluor 405 (A=405, E=421)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Alternate Names for PMEL17/SILV Antibody (HMB45) [Alexa Fluor® 405]

  • D12S53EP1
  • gp100
  • ME20
  • ME20-M
  • melanocyte protein mel 17
  • Melanocyte protein Pmel 17
  • Melanocytes lineage-specific antigen GP100
  • Melanoma-associated ME20 antigen
  • melanosomal matrix protein17
  • PMEL17P100
  • premelanosome proteinME20M
  • SI
  • SIL
  • silver (mouse homolog) like
  • silver homolog (mouse)
  • Silver locus protein homolog
  • silver, mouse, homolog of
  • SILVPmel17

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PMEL17/SILV Antibody (NBP2-34638AF405) (0)

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Secondary Antibodies

 

Isotype Controls

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Diseases for PMEL17/SILV Antibody (NBP2-34638AF405)

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol SILV, PMEL

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