PMEL17/SILV Antibody (HMB45) [FITC]

• Reactivity: Hu, Ca(-), Rt(-)
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready, Func
• Clone: HMB45
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

PMEL17/SILV Antibody (HMB45) [FITC] Summary

• Immunogen: Extract of pigmented melanoma metastases from lymph nodes
• Specificity: The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (6038kDa fragments. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This monoclonal antibody reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This monoclonal antibody stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This monoclonal antibody also stains Angiomyolipoma (PEComa).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PMEL
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Multiplex Immunoassay
  • Western Blot
• Application Notes: Use in Multiplex Immunoassay reported in scientific literature (PMID: 31942075).
  Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Does not react with Canine or Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for PMEL17/SILV Antibody (HMB45) [FITC]

• D12S53EP1
• Gp100
• ME20
• ME20M
• ME20-M
• melanocyte protein mel 17
• Melanocyte protein Pmel 17
• Melanocytes lineage-specific antigen GP100
• Melanoma-associated ME20 antigen
• melanosomal matrix protein17
• PMEL
• PMEL17
• PMEL17P100
• premelanosome proteinME20M
• SI
• SIL
• SILV
• silver (mouse homolog) like
• silver homolog (mouse)
• Silver locus protein homolog
• silver, mouse, homolog of
• SILVPmel17

Background

Melanoma gp100 a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PMEL17/SILV Antibody (NBP2-34638F)(1)

Publication using NBP2-34638F

• Hernandez JOR, Wang X, Vazquez-Segoviano M et al. A tissue-bioengineering strategy for modeling rare human kidney diseases in vivo Nat Commun 2021-11-11 [PMID: 34764250] (FLOW, Human)
  
  Details:
  Citation using the FITC format of this antibody.

Bioinformatics

• Gene Symbol: PMEL