PMEL17/SILV Antibody (HMB45) - Azide and BSA Free

Western Blot: PMEL17/SILV Antibody (HMB45) - Azide and BSA Free [NBP2-34638] - Western Blot Analysis of COLO-38 cell lysate using PMEL17/SILV antibody (HMB45).

Immunohistochemistry: PMEL17/SILV Antibody (HMB45) - Azide and BSA Free [NBP2-34638] - PMEL17 (red) was detected in human skin (melanoma) using PMEL17-PE antibody (1:200) in PBS for 1 hour. Nuclei were stained with DAPI (blue). Image from a verified customer review. Image using the PE format of this antibody.

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (HMB45) - Azide and BSA Free [NBP2-34638] - Formalin-fixed, paraffin-embedded human testis stained with gp100/Melanosome Monoclonal Antibody (HMB45).

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (HMB45) - Azide and BSA Free [NBP2-34638] - Formalin-fixed, paraffin-embedded human melanoma stained with gp100/Melanosome Monoclonal Antibody (HMB45).

• Reactivity: Hu, Ca(-), Rt(-)
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready, Func
• Clone: HMB45
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

PMEL17/SILV Antibody (HMB45) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44520).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Extract of pigmented melanoma metastases from lymph nodes
• Localization: Cytoplasmic
• Marker: Melanoma Marker
• Specificity: The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (6038kDa fragments. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This monoclonal antibody reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This monoclonal antibody stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This monoclonal antibody also stains Angiomyolipoma (PEComa).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PMEL
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/ Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Multiplex Immunoassay
  • Western Blot 0.5-1.0ug/ml
• Application Notes: Use in Multiplex Immunoassay reported in scientific literature (PMID: 31942075).
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
  Use in Western blot reported in scientific literature (PMID: 30561643).
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Does not react with Canine or Rat.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for PMEL17/SILV Antibody (HMB45) - Azide and BSA Free

• D12S53EP1
• Gp100
• ME20
• ME20M
• ME20-M
• melanocyte protein mel 17
• Melanocyte protein Pmel 17
• Melanocytes lineage-specific antigen GP100
• Melanoma-associated ME20 antigen
• melanosomal matrix protein17
• PMEL
• PMEL17
• PMEL17P100
• premelanosome proteinME20M
• SI
• SIL
• SILV
• silver (mouse homolog) like
• silver homolog (mouse)
• Silver locus protein homolog
• silver, mouse, homolog of
• SILVPmel17

Background

Melanoma gp100 a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PMEL17/SILV Antibody (NBP2-34638)(1)

Publication using NBP2-34638

• Elham Karimi, Miranda W. Yu, Sarah M. Maritan, Lucas J. M. Perus, Morteza Rezanejad, Mark Sorin, Matthew Dankner, Parvaneh Fallah, Samuel Doré, Dongmei Zuo, Benoit Fiset, Daan J. Kloosterman, LeeAnn Ramsay, Yuhong Wei, Stephanie Lam, Roa Alsajjan, Ian R. Watson, Gloria Roldan Urgoiti, Morag Park, Dieta Brandsma, Donna L. Senger, Jennifer A. Chan, Leila Akkari, Kevin Petrecca, Marie-Christine Guiot, Peter M. Siegel, Daniela F. Quail, Logan A. Walsh Single-cell spatial immune landscapes of primary and metastatic brain tumours Nature 2023-02-01 [PMID: 36725935]

Bioinformatics

• Gene Symbol: PMEL