PLZF Antibody - BSA Free

Western Blot: PLZF Antibody [NBP2-19870] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PLZF antibody [N1N3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: PLZF Antibody [NBP2-19870] - U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PLZF protein stained by PLZF antibody [N1N3] diluted at 1:2000. Blue: Hoechst 33342 staining.

• Reactivity: Hu, Ch, Mu, Rt, Bv, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PLZF Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PLZF. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (97%), Rat (96%), Rhesus Macaque (98%), Bovine (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZBTB16
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:2000
  • Immunohistochemistry-Frozen Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for PLZF Antibody - BSA Free

• PLZF
• PLZFzinc finger and BTB domain-containing protein 16
• ZBTB16
• zinc finger and BTB domain containing 16
• zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia)
• Zinc finger protein 145
• Zinc finger protein PLZF
• ZNF145
• ZNF145Promyelocytic leukemia zinc finger protein

Background

This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ZBTB16
• Uniprot:
  • Q05516()