PLZF Antibody (5B3)

Western Blot: PLZF Antibody (5B3) [NBP1-51632] - Western blot analysis using ZBTB16 mouse mAb against Hela (1) cell lysate.

Immunocytochemistry/Immunofluorescence: PLZF Antibody (5B3) [NBP1-51632] - Immunofluorescence analysis of Hela cells using ZBTB16 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 5B3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PLZF Antibody (5B3) Summary

• Immunogen: Purified recombinant fragment of human PLZF expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZBTB16
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for PLZF Antibody (5B3)

• PLZF
• PLZFzinc finger and BTB domain-containing protein 16
• ZBTB16
• zinc finger and BTB domain containing 16
• zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia)
• Zinc finger protein 145
• Zinc finger protein PLZF
• ZNF145
• ZNF145Promyelocytic leukemia zinc finger protein

Background

This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZBTB16