PLK1 [p Thr210] Antibody (2A3)

Western Blot: PLK1 [p Thr210] Antibody (2A3) [NBP1-02851] - Western blot using PLK1 [p Thr210] Antibody (2A3)

Immunohistochemistry-Paraffin: PLK1 [p Thr210] Antibody (2A3) [NBP1-02851] - Staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.

Western Blot: PLK1 [p Thr210] Antibody (2A3) [NBP1-02851] - Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with PLK1 [p Thr210] Antibody (2A3) reactive against Thr210-phosphorylated PLK1. Proteins were visualized using an HRP goat anti-mouse secondary and a chemiluminescence detection system.

Product Discontinued

PLK1 [p Thr210] Antibody (2A3) Summary

• Immunogen: Synthetic peptide from human PLK1.
• Modification: p Thr210
• Marker: Mitosis Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 15 ug/ml
  • Immunohistochemistry-Paraffin 15 ug/ml
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2). Sourced from tissue culture supernatant.
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PLK1 [p Thr210] Antibody (2A3)

• cell cycle regulated protein kinase
• EC 2.7.11
• EC 2.7.11.21
• PLK
• PLK1
• PLK-1
• polo (Drosophia)-like kinase
• polo like kinase
• polo-like kinase 1polo-like kinase (Drosophila)
• Serine/threonine-protein kinase 13
• serine/threonine-protein kinase PLK1
• STPK13

Background

PLK, a Polo type protein kinase, has been shown to function in mitosis. The mRNA is absent or expressed at very low levels during the G1 phase, begins to reaccumulate during the S phase, and reaches maximal levels during the G2/M phase. PLK1 may participate in targeting MPF to the nucleus during prophase and in regulating mitotic spindle function. These functions, in addition to its expression in proliferating cells, suggest that PLK1 has a role in cancer progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLK1 Antibody (NBP1-02851)(1)

Publication using NBP1-02851

• Chen J, Yeh C, Cheng C et al. Role of PLK1 signaling pathway genes in gastrointestinal stromal tumors Oncol Lett. [PMID: 30127898] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: PLK1
• Entrez:
  • 5347(Human)
• Uniprot:
  • P53350()