PLGF Antibody (3B10) - Azide and BSA Free

Western Blot: PLGF Antibody (3B10) [NB110-96418] - Figure 1: Western analysis with recombinant human PlGF-1 and PlGF-2 derived from insect cells. The monoclonal mouse anti-human PlGF antibody #3B10 recognizes solely the PlGF-2 isoform.

Immunocytochemistry/Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418] - Immunofluorescence staining of mouse VEGF-A and PLGF in primary mouse endothelial cells (mEC) using a monoclonal antibodies directed against human VEGF-A and human PLGF [NB110-96418], respectively. As secondary antibody goat anti-mouse ALEXA Flour 488 (1:600) was used.

Immunocytochemistry/Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418] - PLGF Antibody (3B10) [NB110-96418] - Immunofluorescence staining of PlGF in a mixture of primary human dermal lymphatic endothelial cells (HDLEC) and human dermal normal fibroblasts with monoclonal mouse anti-human PlGF #3B10. The cells were pretreated with 0,1% Triton X100. Control with goat anti-mouse ALEXA Flour 488.

Immunocytochemistry/Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418] - PLGF Antibody (3B10) [NB110-96418] - Immunofluorescence staining of PlGF in primary human dermal fibroblast cells (HDNF) with monoclonal mouse anti-human PlGF #3B10 (upper panel). Control with goat anti-mouse ALEXA Flour 488 (lower panel).

Immunocytochemistry/Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418] - PLGF Antibody (3B10) [NB110-96418] - Immunofluorescence staining of human PLGF in HUVE cells with the monoclonal mouse anti-human PlGF #3B10 antibody (upper panel). Control with goat anti-mouse ALEXA Flour 488 (lower panel).

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clone: 3B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PLGF Antibody (3B10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PLGF Antibody (3B10) - Azide and BSA Free (NB110-96418) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human PlGF-2
• Specificity: P1GF-2 (3B10)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGF
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1-2 ug/ml
  • Western Blot 1-2 ug/ml

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Alternate Names for PLGF Antibody (3B10) - Azide and BSA Free

• D12S1900
• PGF
• PGFL
• placenta growth factor
• placental growth factor
• placental growth factor, vascular endothelial growth factor-related protein
• PlGF
• PlGF-2
• PLGFplacental growth factor-like
• SHGC-10760

Background

PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It belongs to the cysteine-knot superfamily. PLGF is expressed in placental tissues, colon and mammary carcinomas. It signals through the VEGFR-1/FLT1 receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PGF