PLGF Antibodies

264-PGB) was serially diluted and captured by Mouse Anti-Human PlGF Monoclonal Antibody (Catalog # MAB264) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human PlGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-264-PB) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 264-PGB) was serially diluted and captured by Mouse Anti-Human PlGF Monoclonal Antibody (Catalog # MAB264) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human PlGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-264-PB) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

HAF017). A specific band was detected for PlGF at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for PlGF at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with full length human PlGF. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PlGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-264-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PlGF at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

264-PGB) was serially diluted and captured by Mouse Anti-Human PlGF Monoclonal Antibody (Catalog # MAB264) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human PlGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-264-PB) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 264-PGB) was serially diluted and captured by Mouse Anti-Human PlGF Monoclonal Antibody (Catalog # MAB264) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human PlGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-264-PB) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="PlGF was detected in immersion fixed paraffin-embedded sections of human placenta using Human PlGF Monoclonal Antibody (Catalog # MAB2642) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="PlGF was detected in formalin fixed paraffin-embedded sections of human placenta using Sheep Anti-Human PlGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6837) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="PlGF was detected in immersion fixed MDA‑MB‑231 human breast cancer cell line (positivie staining) and K562 human chronic myelogenous leukemia cell line (negative staining) using Mouse Anti-Human PlGF Monoclonal Antibody (Catalog # MAB2643) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

3516-FL) to Recombinant Human PlGF (264-PGB) immobilized at 0.5 μg/mL (100 µL/well)" class="big_lightbox"> 3516-FL) to Recombinant Human PlGF (264-PGB) immobilized at 0.5 μg/mL (100 µL/well)" alt="In a functional ELISA, 0.150-1.80 µg/mL of this antibody will block 50% of the binding of 100 ng/mL Recombinant Human VEGFR1/Flt-1 Fc Chimera, aa 27-328 (3516-FL) to Recombinant Human PlGF (264-PGB) immobilized at 0.5 μg/mL (100 µL/well)" class="big_thumb" />