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264-PGB) was serially diluted and captured by Mouse Anti-Human PlGF Monoclonal Antibody (Catalog # MAB264) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human PlGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-264-PB) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
HAF017). A specific band was detected for PlGF at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
264-PGB) was serially diluted and captured by Mouse Anti-Human PlGF Monoclonal Antibody (Catalog # MAB264) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human PlGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-264-PB) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It belongs to the cysteine-knot superfamily. PLGF is expressed in placental tissues, colon and mammary carcinomas. It signals through the VEGFR-1/FLT1 receptor.