PlGF-2 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

PlGF-2 Antibody [Biotin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PlGF-2 (R&D Systems, Catalog # 465-PL)
  Ala24-Pro158
  Accession # Q544A5
• Specificity: Detects mouse PlGF-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with rmVEGF R1 Fc Chimera, rmVEGF R2 Fc Chimera, and rmVEGF R3 Fc Chimera is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Pgf
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse PlGF-2 Antibody (Catalog # MAB4651) ELISA Detection: Mouse PlGF-2 Biotinylated Antibody (Catalog # BAF465) Standard: Recombinant Mouse PlGF-2 (Catalog # 465-PL)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PlGF-2 Antibody [Biotin]

• OORS
• PlGF2
• PlGF-2

Background

Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described. In normal mouse tissues, only one mouse PlGF mRNA encoding the equivalent of human PlGF-2 has been identified. Mouse PlGF-2 encodes a 158 amino acid residue precursor protein. Mouse PlGF-2 shares 65% amino acid identity with human PlGF-2. The gene for PlGF has been mapped to mouse chromosome 12 and human chromosome 14. PlGF binds with high-affinity to Flt-1, but not to Flk-1/KDR.

1. DiPalma, T. et al. (1996) Mamm. Genome 7:6.
2. Cao, Y. et al. (1997) Biochem. Biophys. Res. Commun. 235:493.
3. Ferrara, N. et al. (1997) Endocrin. Rev. 18:4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PlGF-2 Antibody (BAF465)(2)

Publications using BAF465

• M Verma, Y Shimizu-Mo, Y Asakura, JP Ennen, J Bosco, Z Zhou, GH Fong, S Josiah, D Keefe, A Asakura Inhibition of FLT1 ameliorates muscular dystrophy phenotype by increased vasculature in a mouse model of Duchenne muscular dystrophy PLoS Genet., 2019-12-26;15(12):e1008468. 2019-12-26 [PMID: 31877123] (ELISA Detection, Mouse)
• Oura H, Bertoncini J, Velasco P, Brown LF, Carmeliet P, Detmar M A critical role of placental growth factor in the induction of inflammation and edema formation. Blood, 2002-09-05;101(2):560-7. 2002-09-05 [PMID: 12393422] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: Pgf
• Uniprot:
  • Q544A5()