Plexin D1: Novus Biologicals

AF4160

Plexin D1 Antibody [Unconjugated]

100 μg

AF4160

HAF017). A specific band was detected for Plexin D1 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of JAR human choriocarcinoma cell line, IMR-32 human neuroblastoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Plexin D1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4160) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for Plexin D1 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of JAR human choriocarcinoma cell line, IMR-32 human neuroblastoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Plexin D1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4160) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Plexin D1 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox">

Human peripheral blood monocytes were stained with Goat Anti-Human Plexin D1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4160, filled histogram) or control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Plexin D1 was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human Plexin D1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4160) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

3239-S3) inhibits proliferation in the HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Plexin D1 mediated inhibition elicited by Recombinant Human Semaphorin 3E (5 ug/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Plexin D1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4160). The ND₅₀ is typically 0.5-2 ug/mL." class="big_lightbox">

Recombinant Human Semaphorin 3E (Catalog # <a class=

3239-S3) inhibits proliferation in the HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Plexin D1 mediated inhibition elicited by Recombinant Human Semaphorin 3E (5 ug/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Plexin D1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4160). The ND₅₀ is typically 0.5-2 ug/mL." class="big_thumb" />

The absence of Mtss1 does not affect medium spiny neuron (MSN) survival, dendritic arborization, and Plexin-D1 expression during striatonigral pathway development.(A) Immunohistochemistry staining for cleaved caspase 3 (CC3) in the striatum of wild-type (WT) or Mtss1 conditional knockout (cKO) mice. The white dotted boxes on the left images are shown in the inset images on the right at a better resolution. Scale bar, 25 μm. (B) Quantification of cell death by the number of CC3-positive cells in a 1 mm2 area covering the dorsal part of the striatum in WT or Mtss1 cKO mice. Error bars, mean ± SEM; ns p>0.05 by Mann‒Whitney test; WT, n = 20; Mtss1 cKO mice, n = 20 (five sections/mouse). (C) Representative images of Golgi staining at low (top panels) and high (bottom panels) magnification. Scale bars, 100 μm. Sholl analysis of dendritic morphology (D) and dendritic length (E) performed by using Neurolucida360 in 3D analysis. Error bars, mean ± SEM; ns p>0.05 by Student’s t-test; WT, n = 12, and Mtss1 cKO mice, n = 15, from three mice. (F, G) Western blot images and quantification of Plexin-D1 expression in the striatum of WT or Mtss1 cKO mice at P5. Error bars, mean ± SEM; *p<0.05 by Student’s t-test; WT mice, n = 3, and Mtss1 cKO mice, n = 4. (H) Plexin-D1 expression in MSNs obtained from the striatum of WT or Mtss1 cKO mice at P0 and measured at DIV6 in culture. (I) Quantification of the western blots shown in (H). Error bars, mean ± SEM; *p<0.05, by Student’s t-test; n = 3 for WT, n = 3 for KO in three independent experiments. Figure 7—figure supplement 3—source data 1.Raw uncropped western blot & gel images. Western blots shown in Figure 7—figure supplement 3F and H.Raw uncropped western blot & gel images. Western blots shown in Figure 7—figure supplement 3F and H. Image collected and cropped by CiteAb from the following open publication (//elifesciences.org/articles/96891), licensed under a CC-BY license. Not internally tested by R&D Systems.

In cultured medium spiny neurons (MSNs), Mtss1 expression is directly regulated by Sema3E-Plexin-D1 signaling through the AKT pathway.(A) WB images showing Mtss1 expression in MSNs derived from the striatum of wild-type (WT) or Plxnd1 conditional knockout (cKO) mice at P0 & measured at DIV3 & DIV6 in culture. (B) Quantification of band intensity in (A). Two-way ANOVA with Tukey’s post hoc correction for multiple comparisons; n = 3. (C) Mtss1 expression in MSNs obtained from the striatum of WT or Sema3e KO mice at P0 & measured at DIV6 in culture. (D) Quantification of the blots shown in (C). Student’s t-test; n = 5 for WT, n = 5 for KO in five independent experiments. (E) Schematic illustration of the experimental strategy for Sema3E-ligand or MK2206, an AKT inhibitor treatment in MSN culture. (F, G) WB images showing Mtss1 expression after AP-Sema3E (2 nM) treatment in cultured MSNs derived from Sema3e KO mice or Plxnd1 cKO mice. (H, I) Quantification of (F, G). Student’s t-test; AP, n = 4, AP-sema3E, n = 4 for sema3e KO mice, AP n = 4, AP-sema3E n = 4 for Plxnd1 cKO mice in three independent experiments. (J, K) WB to analyze the expression of Mtss1 & Plexin-D1 after MK2206 (100 nM) treatment in cultured MSNs & subsequent quantification for band intensity (L, M). Student’s t-test; n = 6 for sham, n = 6 for MK2206 in six independent experiments. (N O) WB image & analysis showing Mtss1 expression in Sema3e knockout MSNs treated with MK2206 after incubation with AP-Sema3E. Two-way ANOVA with Tukey’s post hoc correction for multiple comparisons; n = 5 in five independent experiments. Error bars, mean ± SEM; *p<0.05, **p<0.01, ***p<0.001 by indicated statistical tests. Figure 2—source data 1.WBs shown in Figure 2A, C, F, G, J, K, & N.WBs shown in Figure 2A, C, F, G, J, K, & N. Image collected & cropped by CiteAb from the following open publication (//elifesciences.org/articles/96891), licensed under a CC-BY license. Not internally tested by R&D Systems.

No Mtss1 was found in endothelial cells at E14.5, and no vascular defects were observed in Mtss1-conditional knockout (KO) mice.(A) Fluorescence in situ hybridization for Plxnd1 mRNA (green) and Mtss1 mRNA (red) in the brain and dorsal trunk at E14.5. White dotted boxes are shown in the inset image on the bottom. Scale bars, 100 μm for brain, 50 μm for dorsal trunk. (B) 3D vascular reconstruction analysis images after CD31 immunostaining and tissue clearing obtained using multifunctional fast confocal microscopy Dragonfly 502w. (C) Western blotting to analyze Mtss1 expression after AP-Sema3E (2 nM) treatment in human umbilical vein endothelial cells (HUVECs) or human cortical microvessel endothelial cells (HCMEC/D3). Error bars, mean ± SEM; ns p>0.05 by Mann‒Whitney test; AP, n = 4, AP-Sema3E, n = 4 for HUVECs, ns p>0.05 by Student’s t-test; AP, n = 4, AP-Sema3E, n = 4 for HCMEC/D3s in four dependent experiments. Figure 8—figure supplement 1—source data 1.Western blots shown in Figure 8—figure supplement 1C.Western blots shown in Figure 8—figure supplement 1C. Image collected and cropped by CiteAb from the following open publication (//elifesciences.org/articles/96891), licensed under a CC-BY license. Not internally tested by R&D Systems.

No significant alteration in the expression of vsv-Plexin-D1 or Mtss1-myc or Mtss1 delta I-BAR-myc in the medium spiny neuron (MSN) soma.(A) Representative image of Immunocytochemistry for vsv-Plexin-D1 (red), Mtss1-myc or Mtss1 delta I-BAR -myc (green) in the cell body of cultured MSNs transfected with vsv-Plexin-D1 and Mtss1-myc or Mtss1 delta I-BAR-myc, using Mtss1-null mice as a background. (B) Quantification of the fluorescence intensity in the cell body of (A). Error bars, mean ± SEM; ns p>0.05 by two-way ANOVA with Tukey’s post hoc correction for multiple comparisons; vsv-Plexin-D1+Mtss1 myc, n = 5, and vsv-Plexin-D1+Mtss1 delta I-BAR-myc, n = 5. Scale bar, 5 μm. Image collected and cropped by CiteAb from the following open publication (//elifesciences.org/articles/96891), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of Mtss1 induces I-BAR domain-dependent morphological changes in COS7 cells, generating protrusions.(A) Western blot images showing that weakly expression of endogenous Mtss1 was not altered by overexpression of Plexin-D1 with or without Sema3E in COS7 cells. Asterisk indicates nonspecific band. (B) Quantification of the band intensity in (A). Error bars, mean ± SEM; ns p>0.05 by two-way ANOVA with Bonferroni’s post hoc correction for multiple comparisons; n = 3. (C) Schematics describing the full-length construct of Mtss1-myc and its deletion mutant constructs (Mtss1 delta I-BAR-myc, Mtss1 delta WH2-myc, and I-BAR-myc). (D) Immunocytochemistry images taken after overexpression of each construct. Constructs show the I-BAR domain leading to diverse cell protrusion morphologies. Some of the protrusions were excessively spiked or thin and long (arrowheads). Overexpression of the I-BAR domain only (I-BAR-myc) can induce extreme protrusion structures. Scale bar, 20 μm.Figure 3—figure supplement 1—source data 1.Western blots shown in Figure 3—figure supplement 1A.Western blots shown in Figure 3—figure supplement 1A. Image collected and cropped by CiteAb from the following open publication (//elifesciences.org/articles/96891), licensed under a CC-BY license. Not internally tested by R&D Systems.

Mtss1 expression alters Plexin-D1 localization to the protrusion structure in COS7 cells without affecting its endocytosis or Sema3E binding.(A) Cell surface biotinylation and subsequent Western blot analysis to analyze the surface localization of Plexin-D1 in COS7 cells. Image collected and cropped by CiteAb from the following open publication (//elifesciences.org/articles/96891), licensed under a CC-BY license. Not internally tested by R&D Systems.

Mtss1 facilitates Plexin-D1 transport to the growth cone in cultured Drd1a-positive medium spiny neurons (MSNs).(A) Immunocytochemistry for Mtss1-myc or Mtss1 delta I-BAR -myc (green), vsv-Plexin-D1 (red) in the axons of MSNs transfected with vsv-Plexin-D1 and Mtss1-myc or Mtss1 delta I-BAR-myc, using Mtss1-null mice as a background. The images were acquired using structured illumination microscopy (N-SIM). Scale bar, 5 μm. Image collected and cropped by CiteAb from the following open publication (//elifesciences.org/articles/96891), licensed under a CC-BY license. Not internally tested by R&D Systems.

Mtss1 facilitates Plexin-D1 transport to the growth cone in cultured Drd1a-positive medium spiny neurons (MSNs). Quantification of colocalization by Pearson’s correlation coefficient calculated using Costes’ randomized pixel scrambled image method. Student’s t-test; vsv-Plexin-D1+Mtss1-myc, n = 21, and vsv-Plexin-D1+Mtss1 delta IBAR-myc, n = 14. Image collected and cropped by CiteAb from the following open publication (//elifesciences.org/articles/96891), licensed under a CC-BY license. Not internally tested by R&D Systems.

Sema3E-Plexin-D1 signaling induces Mtss1 expression selectively in developing striatonigral projecting neurons. (K) Western blot images showing the expression of Mtss1 and Plexin-D1 in the striatum of WT or Plxnd1 cKO mice at different developmental stages ranging from embryonic day 16.5 (E16.5) to postnatal day 0 (P0). Image collected and cropped by CiteAb from the following open publication (//elifesciences.org/articles/96891), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF4160U-100UG
Alexa Fluor 405	AF4160V-100UG
Alexa Fluor 488	FAB4160G
Alexa Fluor 532	AF4160X-100UG
Alexa Fluor 594	AF4160T-100UG
Alexa Fluor 647	AF4160R-100UG
Alexa Fluor 700	AF4160N-100UG
Alexa Fluor 750	AF4160S-100UG
Alexa Fluor Plus 405	AF4160AFP405-100UG
Alexa Fluor Plus 488	AF4160AFP488-100UG
Alexa Fluor Plus 555	AF4160AFP555-100UG
Alexa Fluor Plus 594	AF4160AFP594-100UG
Alexa Fluor Plus 647	AF4160AFP647-100UG
Alexa Fluor Plus 680	AF4160AFP680-100UG
Allophycocyanin	AF4160A
Biotin	BAF4160
CoraFluor 1	AF4160CL1
DyLight 350	AF4160D
DyLight 405	AF4160E
DyLight 488	AF4160K
DyLight 550	AF4160L
DyLight 594	AF4160M
DyLight 650	AF4160W
DyLight 680	AF4160Y
DyLight 755	AF4160Z
FITC	AF4160F
HRP	AF4160H
Janelia Fluor 525	AF4160JF525
Janelia Fluor 549	AF4160I
Janelia Fluor 646	AF4160J
Janelia Fluor 669	AF4160JF669
PerCP	AF4160C
Phycoerythrin	FAB4160P
mFluor Violet 450 SE	AF4160MFV450
mFluor Violet 500 SE	AF4160MFV500
mFluor Violet 610 SE	AF4160MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB4160G
Carrier Free	FAB4160P
Carrier Free	AF4160-SP

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MAB41601

Plexin D1 Antibody (752815)

(1 Publication)

100 μg

MAB41601

HAF007). A specific band was detected for Plexin D1 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Plexin D1 Monoclonal Antibody (Catalog # MAB41601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for Plexin D1 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Plexin D1 Monoclonal Antibody (Catalog # MAB41601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Plexin D1 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

<b>Genetic Strategies Validation. </b>Efficient shRNA-mediated knockdown of GIPCs and CRISPR/Cas9-mediated knockout of PLXND1 in HUVEC/TERT2 cells.(A–H) Western blots for GIPC1-2, PLXND1, and GAPDH (loading control) from TCLs of stable cells demonstrating the effective decrease of GIPC1-2 and PLXND1 levels. (A–D) TCLs from non-targeting gRNA#1 cells (A, C) and PLXND1 gRNA KO#1 cells (B, D) infected with the indicated shRNAs and under the different SEMA3E treatments. (E–H) TCLs from non-targeting gRNA#2 cells (E, G) and PLXND1 gRNA KO#2 cells (F, H) infected with the indicated shRNAs and under the different SEMA3E treatments. This figure is related to Figure 7 and Figure 7—figure supplement 1. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31050647), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Genetic Strategies Validation. </b>Efficient shRNA-mediated GIPC (GIPC1, GIPC2, and GIPC3) and PLXND1 knockdowns in primary HUVEC used for cell collapse experiments.Western blots for GIPC1, GIPC2, PLXND1, and GAPDH (loading control) from TCLs of cells infected with the indicated shRNA lentiviral particles. Note the effective decrease of GIPC1-2 and PLXND1 levels. GIPC3 expression was absent under all the experimental conditions assayed. Hence, for brevity, the corresponding Western blots are not shown. This figure is related to Figure 7—figure supplement 3. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31050647), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB41601U
Alexa Fluor 405	FAB41601V-100UG
Alexa Fluor 488	FAB41601G-100UG
Alexa Fluor 532	FAB41601X
Alexa Fluor 594	FAB41601T
Alexa Fluor 647	FAB41601R-100UG
Alexa Fluor 700	FAB41601N-100UG
Alexa Fluor 750	FAB41601S-100UG
Alexa Fluor Plus 405	FAB41601AFP405-100UG
Alexa Fluor Plus 488	FAB41601AFP488-100UG
Alexa Fluor Plus 555	FAB41601AFP555-100UG
Alexa Fluor Plus 594	FAB41601AFP594-100UG
Alexa Fluor Plus 647	FAB41601AFP647-100UG
Alexa Fluor Plus 680	FAB41601AFP680-100UG
Allophycocyanin	FAB41601A
Biotin	FAB41601B
CoraFluor 1	FAB41601CL1
DyLight 350	FAB41601D
DyLight 405	FAB41601E
DyLight 488	FAB41601K
DyLight 550	FAB41601L
DyLight 594	FAB41601M
DyLight 650	FAB41601W
DyLight 680	FAB41601Y
DyLight 755	FAB41601Z
FITC	FAB41601F
HRP	FAB41601H
Janelia Fluor 525	FAB41601JF525
Janelia Fluor 549	FAB41601I
Janelia Fluor 646	FAB41601J
Janelia Fluor 669	FAB41601JF669
PE	FAB41601P
PerCP	FAB41601C
mFluor Violet 450 SE	FAB41601MFV450
mFluor Violet 500 SE	FAB41601MFV500
mFluor Violet 610 SE	FAB41601MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB41601-SP
Carrier Free	FAB41601V-100UG
Carrier Free	FAB41601S-100UG
Carrier Free	FAB41601R-100UG
Carrier Free	FAB41601N-100UG
Carrier Free	FAB41601G-100UG

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NBP1-33634

Plexin D1 Antibody - BSA Free

(2 Publications)

100 ul

NBP1-33634

Immunohistochemistry-Frozen: Plexin D1 Antibody [NBP1-33634] - PlexinD1 on an E12.5 mouse Left Ventricle. Antibody at 1:100. Alexa Fluor 488 secondary antibody at 1:1000. IHC-Fr image submitted by a verified customer review.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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MAB4160

Plexin D1 Antibody (752829)

(1 Publication)

100 μg

MAB4160

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_lightbox">

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." alt="K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Plexin D1 Monoclonal Antibody (Catalog # MAB4160, filled histogram) isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB4160U-100UG
Alexa Fluor 405	FAB4160V
Alexa Fluor 532	FAB4160X
Alexa Fluor 594	FAB4160T-100UG
Alexa Fluor 647	FAB4160R
Alexa Fluor 700	FAB4160N
Alexa Fluor 750	FAB4160S
Alexa Fluor Plus 405	FAB4160AFP405-100UG
Alexa Fluor Plus 488	FAB4160AFP488-100UG
Alexa Fluor Plus 555	FAB4160AFP555-100UG
Alexa Fluor Plus 594	FAB4160AFP594-100UG
Alexa Fluor Plus 647	FAB4160AFP647-100UG
Alexa Fluor Plus 680	FAB4160AFP680-100UG
Allophycocyanin	FAB4160A
Allophycocyanin/Cy7	FAB4160APCCY7
Biotin	FAB4160B
CoraFluor 1	FAB4160CL1
DyLight 350	FAB4160D
DyLight 405	FAB4160E
DyLight 488	FAB4160K
DyLight 550	FAB4160L
DyLight 594	FAB4160M
DyLight 650	FAB4160W
DyLight 680	FAB4160Y
DyLight 755	FAB4160Z
FITC	FAB4160F
HRP	FAB4160H
Janelia Fluor 525	FAB4160JF525
Janelia Fluor 549	FAB4160I
Janelia Fluor 646	FAB4160J
Janelia Fluor 669	FAB4160JF669
PE/Atto594	FAB4160PEATT594
PE/Cy5.5	FAB4160PECY55
PE/Cy7	FAB4160PECY7
PerCP	FAB4160C
mFluor Violet 450 SE	FAB4160MFV450
mFluor Violet 500 SE	FAB4160MFV500
mFluor Violet 610 SE	FAB4160MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB4160-SP
Carrier Free	FAB4160U-100UG
Carrier Free	FAB4160T-100UG

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NB300-961

Plexin D1 Antibody

(2 Publications)

0.1 mg

NB300-961

Immunocytochemistry/Immunofluorescence: Plexin D1 Antibody [NB300-961] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Reactivity:

Hu, Po, Bv, Ca

Species Glossary

Reactivity: Hu, Po, Bv, Ca

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Alt Catalog#

IMG-3783

Alt Catalog# IMG-3783

Conjugate:

Unconjugated

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4160-PD

Recombinant Human Plexin D1 Protein, CF

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP3-12915

Plexin D1 Antibody - BSA Free

100 ul

NBP3-12915

Plexin D1 antibody detects Plexin D1 protein by immunohistochemical analysis.</br>Sample: Frozen-sectioned mouse cerebral cortex.</br>Green: Plexin D1 stained by Plexin D1 antibody (NBP3-12915) diluted at 1:250.</br>Blue: Fluoroshield with DAPI .

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-23327

Plexin D1 Antibody

0.1 ml

NBP3-23327

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-23326

Plexin D1 Antibody

0.1 ml

NBP3-23326

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-23334

Plexin D1 Antibody

0.1 ml

NBP3-23334

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Has Sample Size

Promotional Item

Species Glossary

PLXND1 Products By Gene Name

Plexin D1 Antibody [Unconjugated]

Plexin D1 Antibody (752815)

Plexin D1 Antibody - BSA Free

Plexin D1 Antibody (752829)

Plexin D1 Antibody

Recombinant Human Plexin D1 Protein, CF

Plexin D1 Antibody - BSA Free

Plexin D1 Antibody

Plexin D1 Antibody

Plexin D1 Antibody