Plexin D1 Antibody (752815) [Alexa Fluor® 700]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
752815
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Plexin D1 Antibody (752815) [Alexa Fluor® 700] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Plexin D1
Leu47-Ala1271
Accession # Q9Y4D7
Specificity
Detects human Plexin D1 in ELISAs and Western blots.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PLXND1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plexin D1 Antibody (752815) [Alexa Fluor® 700]

  • KIAA0620MGC75353
  • PLEXD1
  • Plexin D1
  • plexin-D1
  • PLXND1

Background

Plexin D1 is a type I transmembrane glycoprotein that is the prototype of the plexin D subfamily of semaphorin receptors (1, 2). Human Plexin D1 contains a 46 amino acid (aa) signal sequence, a 1225 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 633 aa cytoplasmic domain that includes features common to other plexins (1). The human Plexin D1 ECD shares 89% identity with mouse Plexin D1, and ~84-92% aa identity based on incomplete sequences of rat, bovine, porcine and canine Plexin D1. It contains a sema domain, two plexin-semaphorin-integrin (PSI) or Met-related sequence (MRS) cysteine-rich motifs, and three glycine/proline-rich IPT/TIG domains which are immunoglobulin-like domains found in plexins, transcription factors, and the scatter factor receptors Met and Ron (1, 2). Isoforms of 1787 and 1747 aa have been sequenced; these contain a 178 aa N-terminal deletion with or without a longer alternate C-terminus (3). Like other Sema/plexin interactions, Plexin D1 interacts with Sema3C or Sema4A via neuropilins. Interaction with Sema3E, however, is direct (4). Plexin D1/Sema3E interaction mediates vascular guidance during development or angiogenesis; deletion of either molecule results in similar, profound cardiac abnormalities (4, 5). Plexin D1 is also expressed in lymphocytes, osteoblasts, the neural crest and the central nervous system during development (2, 6). In the brain, the presence of neuropilin can change Plexin D1/Sema3E interaction from an attractive to a repulsive signal (7, 8). Plexin D1 directs migration of thymocytes to the thymic medulla, probably through repulsion of Sema3E (9). Endothelial cell Plexin D1 binding to Sema4A can oppose VEGF and suppresses tumor angiogenesis, and expression of Sema3E correlates inversely with tumor metastasis, indicating that Plexin D1 is anti-metastatic in the presence of its ligands (10, 11).

  1. Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
  2. Van Der Zwaag, B. et al. (2002) Dev. Dyn. 225:336.
  3. Entrez protein Accession # Q9Y4D7, EAW79239, EAW79240.
  4. Gu, C. et al. (2005) Science 307:265.
  5. Gitler, A.D. et al. (2004) Developmental Cell 7:107.
  6. Zhang, Y. et al. (2009) Dev. Biol. 325:82.
  7. Chauvet, S. et al. (2007) Neuron 56:807.
  8. Pecho-Vrieseling, E. et al. (2009) Nature 459:842.
  9. Choi, Y.I. et al. (2008) Immunity 29:888.
  10. Toyofuku, T. et al. (2007) EMBO J. 26:1373.
  11. Roodink, I. et al. (2008) Am. J. Pathol. 173:1873.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PLXND1
Uniprot