ARL5B Antibody (OTI2D7)

Western Blot: ARL5B Antibody (2D7) [NBP2-01669] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARL5B.

Immunocytochemistry/Immunofluorescence: ARL5B Antibody (2D7) [NBP2-01669] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ARL5B.

Immunohistochemistry-Paraffin: ARL5B Antibody (2D7) [NBP2-01669] - Staining of paraffin-embedded Human colon tissue using anti-ARL5B mouse monoclonal antibody.

Flow Cytometry: ARL5B Antibody (2D7) [NBP2-01669] - Analysis of Jurkat cells, using anti-ARL5B antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI2D7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.62 mg/ml

ARL5B Antibody (OTI2D7) Summary

• Description: Novus Biologicals Mouse ARL5B Antibody (OTI2D7) (NBP2-01669) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ARL5B(NP_848930) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARL5B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 20.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.62 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARL5B Antibody (OTI2D7)

• ADP-ribosylation factor-like 5B
• ADP-ribosylation factor-like 8
• ADP-ribosylation factor-like protein 5B
• ADP-ribosylation factor-like protein 8
• ADP-ribosylation-like factor 8
• ARL8

Background

ARL5B (ARL8) belongs to a family of proteins that are structurally similar to ADP-ribosylation factors (ARFs; see MIM 103180). ARLs and ARFs are part of the RAS superfamily of regulatory GTPases.[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARL5B
• Entrez:
  • 221079(Human)
• Uniprot:
  • Q96KC2(Human)