PKN1 Antibody (540421) [Alexa Fluor® 700]

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 540421
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

PKN1 Antibody (540421) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human PKN1
  Ile215-Thr388
  Accession # Q16512
• Specificity: Detects human and mouse PKN1 in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PKN1 Antibody (540421) [Alexa Fluor® 700]

• DBK
• EC 2.7.11
• MGC46204
• PAK1
• PAK1Serine-threonine protein kinase N
• PKN
• PKN1
• PKN-ALPHA
• PKNPKN-ALPHA
• PRK1
• PRK1PAK-1
• PRKCL1
• PRKCL1protein kinase C-related kinase 1
• Protease-activated kinase 1
• Protein kinase C-like 1EC 2.7.11.13
• Protein kinase C-like PKN
• protein kinase N1
• Protein kinase PKN-alpha
• Protein-kinase C-related kinase 1
• serine/threonine protein kinase N
• serine/threonine-protein kinase N1
• serine-threonine kinase N

Background

PKN1 (serine/threonine protein kinase N1), also known as PKN or PRK, is a ubiquitous 120 kDa cytoplasmic kinase of the PKC superfamily. The 942 amino acid (aa) PKN1 contains three HR1 repeats (aa 34‑263), a C2 domain (aa 325‑461), and a protein kinase domain (aa 615‑874). PKN1 can be activated by proteolysis, lipid interaction, or Rho G proteins, and is a downstream effector of RhoA. It promotes the activation of several kinases involved in regulation of the actin cytoskeleton, cell cycle, and apoptosis. In vivo, PKN1 phosphorylates histone H3 at the Thr11 position.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.