PKM2 Antibody (7B2) - BSA Free

Western Blot: PKM2 Antibody (7B2) [NBP3-15230] - Positive WB detected in: SH-SY5Y whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse brain tissue. All lanes: PKM2 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58, 59, 57 KDa. Observed band size: 58 KDa.

Immunocytochemistry/Immunofluorescence: PKM2 Antibody (7B2) [NBP3-15230] - Immunofluorescence staining of Hela Cells with at PKM2 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry: PKM2 Antibody (7B2) [NBP3-15230] - IHC image of PKM2 antibody diluted at 1:100 and staining in paraffin-embedded human lung cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Flow Cytometry: PKM2 Antibody (7B2) [NBP3-15230] - Overlay histogram showing HepG2 cells stained with PKM2 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunoprecipitation: PKM2 Antibody (7B2) [NBP3-15230] - Immunoprecipitating PKM2 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of PKM2 antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PKM2 antibody (2ug)+ Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP
• Clone: 7B2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PKM2 Antibody (7B2) - BSA Free Summary

• Description: Novus Biologicals Rabbit PKM2 Antibody (7B2) - BSA Free (NBP3-15230) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from human PKM2. UniProt ID (P14618).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PKM
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PKM2 Antibody (7B2) - BSA Free

• CTHBP
• Cytosolic thyroid hormone-binding protein
• MGC3932
• OIP3
• OIP-3
• OIP3EC 2.7.1.40
• Opa-interacting protein 3
• p58
• PK, muscle type
• PK2
• PK3
• PK3PKM
• PKM2
• Pyruvate kinase 2/3
• pyruvate kinase isozymes M1/M2
• Pyruvate kinase muscle isozyme
• pyruvate kinase, muscle
• TCB
• THBP1
• THBP1p58
• Thyroid hormone-binding protein 1
• thyroid hormone-binding protein, cytosolic
• Tumor M2-PK

Background

PKM2 is encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PKM