PKC zeta Antibody - BSA Free

Western Blot: PKC zeta Antibody [NB100-82147] - Analysis of extract from 293 cells and Hela using PKC zeta antibody.

Immunohistochemistry-Paraffin: PKC zeta Antibody [NB100-82147] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NB100-82147.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PKC zeta Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PKC zeta Antibody - BSA Free (NB100-82147) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-PKC zeta Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide sequence around amino acids 408~412 ( T-S-T-F-C) derived from PKC zeta
• Specificity: Detects endogenous levels of total PKC zeta protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKCZ
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27064903).
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PKC zeta Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.13
• nPKC-zeta
• PKC zeta
• PKC2
• PKC2PKC-ZETA
• PRKCZ
• protein kinase C zeta type
• protein kinase C, zeta

Background

Protein Kinase C (PKC) isoforms are serine/threonine kinases involved in signal transduction pathways that govern a wide range of physiological processes including differentiation, proliferation, gene expression, brain function, membrane transport and the organization of cytoskeletal and extracellular matrix proteins. Increasing evidence from studies using in vitro and in vivo systems points to PKC as a key regulator of critical cell cycle transitions, including cell cycle entry and exit and the G1 and G2 checkpoints. PKC-mediated control of these transitions can be negative or positive, depending on the timing of PKC activation during the cell cycle and on the specific PKC isozymes involved. There have been at least 12 different PKC isoforms identified in humans to date, including alpha, beta I, beta II, gamma, delta, epsilon, zeta, eta, theta, iota, lambda, and mu.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PKC zeta Antibody (NB100-82147)(1)

Publication using NB100-82147

• Verbist KC, Guy CS, Milasta S et al. Metabolic maintenance of cell asymmetry following division in activated T lymphocytes. Nature. 2016-04-21 [PMID: 27064903] (ICC/IF, Mouse)

Review for PKC zeta Antibody (NB100-82147) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 11/05/2017

Summary

• Application: Western Blot
• Sample Tested: U266 human myeloma cell line
• Species: Human
• Lot: 8715

Bioinformatics

• Gene Symbol: PRKCZ
• Entrez:
  • 5590(Human)
  • 18762(Mouse)
  • 25522(Rat)
• Uniprot:
  • Q05513()