PKC zeta Antibody

Images

 
Western Blot: PKC zeta Antibody [NB100-82147] - Analysis of extract from 293 cells and Hela using PKC zeta antibody.
Immunohistochemistry-Paraffin: PKC zeta Antibody [NB100-82147] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKC zeta antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

PKC zeta Antibody Summary

Immunogen
Peptide sequence around aa. 408~412 ( T-S-T-F-C) derived from PKCz
Specificity
PKC zeta (specific to a.a. 410) detects endogenous levels of total PKC protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKCZ
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27064903).
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB100-82147 in the following applications:

Publications
Read Publication using
NB100-82147 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PKC zeta Antibody

  • EC 2.7.11
  • EC 2.7.11.13
  • nPKC-zeta
  • PKC zeta
  • PKC2
  • PKC2PKC-ZETA
  • PRKCZ
  • protein kinase C zeta type
  • protein kinase C, zeta

Background

Protein Kinase C (PKC) isoforms are serine/threonine kinases involved in signal transduction pathways that govern a wide range of physiological processes including differentiation, proliferation, gene expression, brain function, membrane transport and the organization of cytoskeletal and extracellular matrix proteins. Increasing evidence from studies using in vitro and in vivo systems points to PKC as a key regulator of critical cell cycle transitions, including cell cycle entry and exit and the G1 and G2 checkpoints. PKC-mediated control of these transitions can be negative or positive, depending on the timing of PKC activation during the cell cycle and on the specific PKC isozymes involved. There have been at least 12 different PKC isoforms identified in humans to date, including alpha, beta I, beta II, gamma, delta, epsilon, zeta, eta, theta, iota, lambda, and mu.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PKC zeta Antibody (NB100-82147)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Mouse
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Review for PKC zeta Antibody (NB100-82147) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-82147:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot PKC zeta NB100-82147
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reviewed by:
Anonymous
WB Human 11/05/2017
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Summary

ApplicationWestern Blot
Sample TestedU266 human myeloma cell line
SpeciesHuman
Lot8715

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PKC zeta Antibody (NB100-82147) (0)

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Secondary Antibodies

 

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Recent Reviews

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1
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Anonymous
11/05/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol PRKCZ
Entrez
Uniprot