PKC zeta [p Thr560] Antibody (SR2127) - BSA Free

Western Blot: PKC zeta [p Thr 560] Antibody (SR2127) [NBP3-21553] - Western blot analysis using NBP3-21553 in HeLa cell treated with Calyculin A lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: SR2127
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PKC zeta [p Thr560] Antibody (SR2127) - BSA Free Summary

• Description: Novus Biologicals Rabbit PKC zeta [p Thr560] Antibody (SR2127) - BSA Free (NBP3-21553) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human phospho-PKC zeta [Thr560] (Uniprot #: Q05513)
• Modification: p Thr560
• Specificity: Detects endogenous levels of phospho-PKC zeta [Thr560]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKCZ
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:1000-1:2000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PKC zeta [p Thr560] Antibody (SR2127) - BSA Free

• EC 2.7.11
• EC 2.7.11.13
• nPKC-zeta
• PKC zeta
• PKC2
• PKC2PKC-ZETA
• PRKCZ
• protein kinase C zeta type
• protein kinase C, zeta

Background

Protein kinase C (PKC), a class of serine/threonine kinases activated by Ca2+ and/or phospholipids, is involved in a variety of cellular processes such as proliferation, differentiation and secretion. PKC zeta exhibits a constitutive kinase activity which is independent of Ca2+, phosphatidylserine and diacylglycerol. Arachidonic acid alone or a combination of gamma-linolenic acid and phosphatidylserine slightly enhance PKC zeta activity. PKC zeta, in contrast to PKC alpha, is insensitive to PKC inhibitors known to interfere either with the regulatory or the catalytic domain and cannot be activated by phorbol ester treatment of NIH 3T3 cells or insect cells, overexpressing the respective PKC isoenzyme (1). Results have identified PDK-1 as the kinase that phosphorylates and activates PKC zeta in the PI 3-kinase signaling pathway. This phosphorylation and activation of PKC zeta by PDK-1 is enhanced in the presence of Ptdins-3,4-5-P3. PKCs are enzymes that are regulated at the plasma membrane, and a membrane-targeted PKC zeta is constitutively active in the absence of agonist stimulation. The association between PKC zeta and PDK-1 reveals extensive cross-talk between enzymes in the PI 3-kinase signaling pathway. (2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKCZ