PKC zeta Antibody (R14-K)

Western Blot: PKC zeta Antibody (R14-K) [NBP1-30127] - Lane 1-50ng of recombinant human PKC-zeta (N-term GST PKC-zeta; BML-SE443-0005); lane 2-mouse brain crude protein lysate (100 ug); lane 3-rat brain crude.

Immunocytochemistry/Immunofluorescence: PKC zeta Antibody (R14-K) [NBP1-30127] - Expression in HEK293 cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IP
• Clone: R14-K
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PKC zeta Antibody (R14-K) Summary

• Description: This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
• Immunogen: Peptide derived from internal sequence on human PKC zeta. Antibody recognizes the epitope located between Lys220 - Leu231
• Epitope: Lys220 - Leu231
• Specificity: This antibody is specific for PKC-Zeta.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKCZ
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:100000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunoprecipitation
  • Western Blot 1:5000
• Application Notes: Dilutions for IP must be determined by end user.
• Control:

  Human Brain Cerebral Cortex Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Alternate Names for PKC zeta Antibody (R14-K)

• EC 2.7.11
• EC 2.7.11.13
• nPKC-zeta
• PKC zeta
• PKC2
• PKC2PKC-ZETA
• PRKCZ
• protein kinase C zeta type
• protein kinase C, zeta

Background

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PKC zeta Antibody (NBP1-30127)(1)

Publication using NBP1-30127

• Cai-McRae X, Zhong H, Karantza V. Sequestosome 1/p62 facilitates HER2-induced mammary tumorigenesis through multiple signaling pathways. Oncogene. 2014-08-04 [PMID: 25088198]

Bioinformatics

• Gene Symbol: PRKCZ
• Entrez:
  • 5590(Human)